{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:30:20Z","timestamp":1788402620457,"version":"build-2803163510"},"reference-count":64,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,10,24]],"date-time":"2007-10-24T00:00:00Z","timestamp":1193184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We examined neurodegeneration in spinal cord (SC) and role of such extra\u2010nigral degeneration in MPTP\u2010induced experimental parkinsonism in C57BL\/6N mice. HPLC\u2010photodiode array analysis confirmed presence of the active neurotoxin MPP<jats:sup>+<\/jats:sup>in SC after single injection of MPTP (25\u2003mg\/kg, i.p.). Mitochondrial enzyme monoamine oxidase\u2010B (MAO\u2010B) responsible for<jats:italic>in vivo<\/jats:italic>conversion of MPTP to MPP<jats:sup>+<\/jats:sup>was inhibited in SC by pre\u2010treatment with<jats:sc>l<\/jats:sc>\u2010deprenyl, a specific inhibitor of MAO\u2010B. Besides<jats:italic>in vitro<\/jats:italic>conversion of MPTP to MPP<jats:sup>+<\/jats:sup>occurred by SC mitochondrial preparation, which was inhibited by<jats:sc>l<\/jats:sc>\u2010deprenyl implicating SC as a specific target of MPTP\u2010neurotoxicity. Double immunofluorescent labeling and spectrofluorimetric assay via kynuramine oxidation showed MAO\u2010B expression and activity in SC neurons. Localization of dopamine transporter immunoreactivity in SC along with specific uptake of<jats:sup>3<\/jats:sup>H\u2010MPP<jats:sup>+<\/jats:sup>by SC synaptosomal preparation further confirmed SC as target of MPTP\u2010neurotoxicity. Compared with control, increased neuronal death on the seventh day in SC of mice injected with MPTP (2\u2003\u00d7\u200325\u2003mg\/kg, at 6\u2003h interval) strongly suggested SC degeneration in pre\u2010symptomatic phase of MPTP\u2010induced experimental parkinsonism. Such extra\u2010nigral neurodegeneration in Parkinson\u2019s disease indicated novel molecular mechanism preceding nigrostriatal degeneration and suggested designing broad therapeutic intervention for this complex movement disorder.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2007.05091.x","type":"journal-article","created":{"date-parts":[[2007,11,28]],"date-time":"2007-11-28T00:54:00Z","timestamp":1196211240000},"page":"1309-1320","source":"Crossref","is-referenced-by-count":25,"title":["Spinal cord degeneration in C57BL\/6N mice following induction of experimental parkinsonism with MPTP"],"prefix":"10.1111","volume":"104","author":[{"given":"Supriti","family":"Samantaray","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Varduhi H.","family":"Knaryan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan T.","family":"Butler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swapan K.","family":"Ray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naren L.","family":"Banik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,10,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3831-03.2004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2006.093849"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1998.6995"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.2006.00727.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-4580(02)00065-9"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.14.4546"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.43.11.2258"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2002.tb04169.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2004.01.065"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(84)91293-2"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(84)90221-8"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-10-04081.2003"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00568-3"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1781(79)90006-4"},{"key":"e_1_2_7_16_1","first-page":"691","article-title":"Correlation between the neostriatal content of the 1\u2010methyl\u20104\u2010phenylpyridinium species and dopaminergic neurotoxicity following 1\u2010methyl\u20104\u2010phenyl\u20101,2,3,6\u2010tetrahydropyridine administration to several strains of mice","volume":"257","author":"Giovanni A.","year":"1991","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_17_1","first-page":"1000","article-title":"Studies on species sensitivity to the dopaminergic neurotoxin 1\u2010methyl\u20104\u2010phenyl\u20101,2,3,6\u2010tetrahydropyridine. Part 1: Systemic administration","volume":"270","author":"Giovanni A.","year":"1994","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/35081564"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410370604"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903620308"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(84)90521-1"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(89)90427-X"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19971117)388:2<211::AID-CNE3>3.0.CO;2-4"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19961223)376:4<631::AID-CNE10>3.0.CO;2-P"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-8043(01)00253-6"},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/S0022-3565(24)35176-6","article-title":"Species\u2010dependent differences in monoamine oxidase A and B\u2010catalyzed oxidation of various C4 substituted 1\u2010methyl\u20104\u2010phenyl\u20101,2,3, 6\u2010tetrahydropyridinyl derivatives","volume":"291","author":"Inoue H.","year":"1999","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.342"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(84)90740-4"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.7.2173"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(93)90056-Z"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000204179.88955.fa"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6823561"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.19.9074"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0773.1985.tb00020.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/311464a0"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4308-05.2006"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1986.tb00722.x"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)91141-8"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0160-5402(85)90021-X"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(96)00100-5"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.10239"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(85)90146-8"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410260606"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2003.tb07476.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00183.x"},{"key":"e_1_2_7_46_1","doi-asserted-by":"crossref","first-page":"7585","DOI":"10.1016\/S0021-9258(19)57434-8","article-title":"Energy\u2010dependent uptake of N\u2010methyl\u20104\u2010phenylpyridinium, the neurotoxic metabolite of 1\u2010methyl\u20104\u2010phenyl\u20101,2,3,6\u2010tetrahydropyridine, by mitochondria","volume":"261","author":"Ramsay R. R.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb05202.x"},{"key":"e_1_2_7_48_1","first-page":"305","article-title":"Combined TUNEL and double immunofluorescent labeling for detection of apoptotic mononuclear phagocytes in autoimmune demyelinating disease","volume":"5","author":"Ray S. K.","year":"2000","journal-title":"Brain Res."},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.22.8160"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1996.tb01294.x"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002826-199004000-00006"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(03)00577-9"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(03)00238-6"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1369.034"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2007.01.056"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.3109\/00207458709043349"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)92366-0"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(86)90340-9"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(97)00570-8"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/42166"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00702-004-0203-2"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(91)90076-6"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(84)90112-7"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(87)90857-2"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)01394-2"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1471-4159.2007.05091.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1471-4159.2007.05091.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T00:01:28Z","timestamp":1737590488000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1471-4159.2007.05091.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10,24]]},"references-count":64,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2008,3]]}},"alternative-id":["10.1111\/j.1471-4159.2007.05091.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1471-4159.2007.05091.x","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,10,24]]}}}