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Retinal cells isolated at embryonic day 8 (E8) and cultured for 3\u2003days in serum\u2010supplemented medium differentiated mostly into red\u2010sensitive cones and to a lesser degree into green\u2010sensitive cones, as indicated by real\u2010time RT\u2010PCR quantification of each specific opsin mRNA. In contrast, both rhodopsin mRNA concentration and rhodopsin gene promoter activity required the presence of cAMP\u2010increasing agents [forskolin and 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX)] to reach significant levels. This response was rod\u2010specific and was sufficient to activate rhodopsin gene transcription in serum\u2010free medium. The increase in rhodopsin mRNA levels evoked by a series of cAMP analogs suggested the response was mediated by protein kinase A, not by EPAC. Membrane depolarization by high KCl concentration also increased rhodopsin mRNA levels and this response was strongly potentiated by IBMX. The rhodopsin gene response to cAMP\u2010increasing agents was developmentally gated between E6 and E7. Rod\u2010specific transducin \u03b1 subunit mRNA levels also increased up to 50\u2010fold in response to forskolin and IBMX, while rod\u2010specific phosphodiesterase\u2010VI and rod arrestin transcripts increased 3\u2010 to 10\u2010fold. These results suggest a cAMP\u2010mediated signaling pathway may play a role in rod differentiation.<\/jats:p>","DOI":"10.1111\/j.1471-4159.2009.06136.x","type":"journal-article","created":{"date-parts":[[2009,4,30]],"date-time":"2009-04-30T14:35:36Z","timestamp":1241102136000},"page":"318-327","source":"Crossref","is-referenced-by-count":7,"title":["Cyclic AMP\u2010dependent activation of rhodopsin gene transcription in cultured retinal precursor cells of chicken embryo"],"prefix":"10.1111","volume":"110","author":[{"given":"Pierre","family":"Voisin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Bernard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,6,15]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"134","volume-title":"Methods in Neuroscience","author":"Adler R.","year":"1990"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(00)00008-2"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.03.044"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0331"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00109.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2002.01162.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.0220"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.05150.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2900661"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.07.002"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-04-01430.1996"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0192-0561(98)00041-1"},{"key":"e_1_2_7_14_1","first-page":"472","article-title":"Differential regulation of melatonin synthesis genes and phototransduction genes in embryonic chicken retina and cultured retinal precursors cells","volume":"11","author":"Cailleau V.","year":"2005","journal-title":"Mol. 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