{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:42:25Z","timestamp":1791301345525,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,8,31]],"date-time":"2010-08-31T00:00:00Z","timestamp":1283212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:sec><jats:label\/><jats:p> <jats:italic>J. Neurochem.<\/jats:italic> (2010) <jats:bold>115<\/jats:bold>, 537\u2013549.<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Japanese encephalitis virus (JEV), the leading cause of acute encephalitis in South\u2010East Asia is a neurotropic virus infecting various CNS cell types. Most Flaviviruses including JEV get internalised into cells by receptor\u2010mediated endocytosis, which involve clathrin and membrane cholesterol. The cholesterol\u2010enriched membrane microdomains referred to as lipid rafts act as portals for virus entry in a number of enveloped viruses, including Flavivirus. However, the precise role played by membrane lipid rafts in JEV internalisation into neural stem cells is still unknown. We have established neural stem\/progenitor cells and C17.2 cell line as models of productive JEV infection. Increase in membrane fluidity and clustering of viral envelope proteins in lipid rafts was observed in early time points of infection. Localisation of non\u2010structural proteins to rafts at later infection stages was also observed. Co\u2010localisation of JEV glycoprotein with Cholera toxin B confirmed that JEV internalisation occurs in a lipid\u2010raft dependent manner. Though JEV entry is raft dependent, however, there is requirement of functional clathrin during endocytosis inside the cells. Besides virus entry, the lipid rafts act as signalling platforms for Src tyrosine kinases and result in activation of phosphoinosit\u00ecde 3\u2032\u2010kinase\/Akt signalling during early JEV infection. Disruption of lipid raft formation by cholesterol depletion using Methyl \u03b2\u2010cyclodextrin, reduced JEV RNA levels and production of infectious virus particles as well as impaired phosphoinosit\u00ecde 3\u2032\u2010kinase\/Akt signalling during initial infection. Overall, our results implicate the importance of host membrane lipid rafts in JEV entry and life cycle, besides maintaining survival of neural stem\/progenitor cells during early infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1471-4159.2010.06951.x","type":"journal-article","created":{"date-parts":[[2010,8,13]],"date-time":"2010-08-13T00:30:18Z","timestamp":1281659418000},"page":"537-549","source":"Crossref","is-referenced-by-count":90,"title":["Critical role of lipid rafts in virus entry and activation of phosphoinositide 3\u2032 kinase\/Akt signaling during early stages of Japanese encephalitis virus infection in neural stem\/progenitor cells"],"prefix":"10.1111","volume":"115","author":[{"given":"Sulagna","family":"Das","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swarupa","family":"Chakraborty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anirban","family":"Basu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,8,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83357-0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(03)59007-8"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2008.08.008"},{"issue":"9","key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1242\/jcs.112.9.1303","article-title":"Inhibition of clathrin\u2010coated pit assembly by an Eps15 mutant","volume":"112","author":"Benmerah A.","year":"1999","journal-title":"J. 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