{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T11:48:25Z","timestamp":1791028105216,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,5,13]],"date-time":"2011-05-13T00:00:00Z","timestamp":1305244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:sec><jats:label\/><jats:p><jats:italic>J. Neurochem.<\/jats:italic>(2011)<jats:bold>117<\/jats:bold>, 1047\u20131054.<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Protein phosphatase PP2A dephosphorylates phosphorylated tau (P\u2010tau) and neurofilaments (pNFs). PP2A is<jats:italic>S<\/jats:italic>\u2010adenosylmethionine (SAM)\u2010dependent and might thus link methylation with neurodegeneration. Low SAM and increased<jats:italic>S<\/jats:italic>\u2010adenosylhomocysteine (SAH) can enhance the risk of dementia. We studied the effect of hyperhomocysteinemia on P\u2010tau (Ser396), pNF\u2010H (heavy chain), and PP2A\u2010activity and level (the C subunit) in rat brain. Wistar rats (total<jats:italic>n\u2003<\/jats:italic>=\u200355) were fed either on a standard, a homocystine 1.7% or a methionine 2.4%\u2010rich diet for 5\u2003months. P\u2010tau was tested in 21 frontal cortex tissue slices using immuno\u2010fluorescence. Concentrations of pNF\u2010H and the activity and level of PP2A were measured in brain extracts. Concentrations of homocysteine, SAM and SAH strongly increased in plasma of rats on the modified diets. The diets caused lowering of plasma folate and vitamin B12 and a significant increase in P\u2010tau (Ser396) in brain tissues but PP2A activity and level were unchanged. Plasma folate correlated to brain tissue PP2A activity (<jats:italic>r<\/jats:italic>\u2003=\u20030.28), pNF\u2010H (<jats:italic>r<\/jats:italic>\u2003=\u2003<jats:bold>\u2212<\/jats:bold>0.30), and P\u2010tau (Ser396) staining (<jats:italic>r<\/jats:italic>\u2003=\u2003<jats:bold>\u2212<\/jats:bold>0.57) all<jats:italic>p\u2003<\/jats:italic>&lt;\u20030.05. Phosphorylation of brain functional proteins was related to folate. The effect of the diet on P\u2010tau and pNF\u2010H seemed not to be explained by a lower activity or protein level of PP2A. Folate might prove protective against multiple steps in the process of neurodegeneration.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1471-4159.2011.07280.x","type":"journal-article","created":{"date-parts":[[2011,4,21]],"date-time":"2011-04-21T17:00:45Z","timestamp":1303405245000},"page":"1047-1054","source":"Crossref","is-referenced-by-count":19,"title":["Folate is related to phosphorylated neurofilament\u2010H and P\u2010tau (Ser396) in rat brain"],"prefix":"10.1111","volume":"117","author":[{"given":"Rima","family":"Obeid","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Schlundt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Umanskaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Herrmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Herrmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,5,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.10251"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.1.298"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2007.0488"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0404.1994.tb05405.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.01.008"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"483","DOI":"10.3233\/JAD-2009-1076","article-title":"Dietary deficiency in folate and vitamin E under conditions of oxidative stress increases phospho\u2010tau levels: potentiation by ApoE4 and alleviation by S\u2010adenosylmethionine","volume":"17","author":"Chan A.","year":"2009","journal-title":"J. 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