{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:49:50Z","timestamp":1722559790246},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,7,21]],"date-time":"2011-07-21T00:00:00Z","timestamp":1311206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:sec><jats:label \/><jats:p><jats:italic>J. Neurochem.<\/jats:italic> (2011) <jats:bold>118<\/jats:bold>, 737\u2013748.<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Protein kinase C\u2010\u03b6 interacting proteins (ZIP1\u20103) recruit the enzymatic activity of the atypical protein kinase C isoforms PKC\u2010\u03bb\/\u03b9 or PKC\u2010\u03b6 to target proteins. In this study, we searched for binding partners of ZIP3 in the CNS and identified spartin, a multifunctional protein that is mutated in spastic paraplegia type 20. In transfected cells, spartin was present on the surface of lipid droplets (LD), whereas ZIP proteins appeared in intracellular speckles. In the presence of spartin, ZIP1 and ZIP3 were translocated to spartin\u2010positive LD. This translocation was mediated by amino acids 196\u2013393 of spartin that interacted with an N\u2010terminal region of ZIP proteins. Furthermore, ZIP proteins interacted simultaneously with spartin and PKC\u2010\u03b6, resulting in an enrichment of PKC\u2010\u03b6 on spartin\/ZIP\u2010labelled LD. Without spartin, neither ZIP proteins nor PKC\u2010\u03b6 were detected on LD. Interestingly, the presence of the spartin\/ZIP\/PKC\u2010\u03b6 complex increased LD size. This effect was most pronounced upon incorporation of the ZIP3 isoform into the trimer. Finally, we co\u2010localized spartin, ZIP proteins and PKC\u2010\u03b6 in axon terminals of neurons in the mammalian retina. In summary, we describe spartin as new binding partner of the ZIP\/PKC\u2010\u03b6 dimer that recruits PKC\u2010\u03b6 to LD and show that the expressed ZIP isoform regulates LD size.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1471-4159.2011.07367.x","type":"journal-article","created":{"date-parts":[[2011,6,30]],"date-time":"2011-06-30T11:02:48Z","timestamp":1309431768000},"page":"737-748","source":"Crossref","is-referenced-by-count":11,"title":["Spartin recruits PKC\u2010\u03b6 via the PKC\u2010\u03b6\u2010interacting proteins ZIP1 and ZIP3 to lipid droplets"],"prefix":"10.1111","volume":"118","author":[{"given":"Andreas","family":"Urbanczyk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Enz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,7,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02941"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.06.201"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.65.4.520"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr070158j"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.03.022"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200507002"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2005.08.009"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0888-7543(03)00011-9"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205162200"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200808041"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20082398"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.1999.00423.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","first-page":"4479","DOI":"10.1523\/JNEUROSCI.16-14-04479.1996","article-title":"Immunocytochemical localization of the GABAc receptor rho subunits in the mammalian retina","volume":"16","author":"Enz R.","year":"1996","journal-title":"J. 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