{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T15:32:43Z","timestamp":1757777563873},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,1,23]],"date-time":"2012-01-23T00:00:00Z","timestamp":1327276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:sec><jats:label \/><jats:p><jats:italic>J. Neurochem<\/jats:italic> (2012) <jats:bold>120<\/jats:bold>, 699\u2013709.<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>L1 cell adhesion molecule (L1CAM), an adhesion\/signaling protein encoded by a gene target of the transcription repressor RE\u20101\u2010Silencing Transcription factor (REST), is expressed in two alternatively spliced isoforms. The full\u2010length isoform, typical of low\u2010REST neural cells, plays key roles in survival\/migration, outgrowth\/fasciculation\/regeneration of axons, synaptic plasticity; the isoform missing two mini\u2010exons, abundant in a few high\u2010REST non\u2010neural cells, maintains some effect on migration and proliferation. To investigate whether and how L1CAM alternative splicing depends on REST we used neural cell models expressing low or high levels of REST (PC12, SH\u2010SY5Y, differentiated NT2\/D1 and primary neurons transduced or not with REST). The short isoform was found to rise when the low\u2010REST levels of neural cells were experimentally increased, while the full\u2010length isoform increased in high\u2010REST cells when the repressor tone was attenuated. These results were due to Nova2, a neural cell\u2010specific splicing factor shown here to be repressed by REST. REST control of L1CAM occurs therefore by two mechanisms, transcription and alternative splicing. The splicing mechanism, affecting not only L1CAM but all Nova2 targets (\u223c7% of brain\u2010specific splicing, including the mRNAs of other adhesion and synaptic proteins) is expected to be critical during development and important also for the structure and function of mature neural cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1471-4159.2011.07626.x","type":"journal-article","created":{"date-parts":[[2011,12,16]],"date-time":"2011-12-16T16:23:57Z","timestamp":1324052637000},"page":"699-709","source":"Crossref","is-referenced-by-count":16,"title":["Dual REST\u2010dependence of L1CAM: from gene expression to alternative splicing governed by Nova2 in neural cells"],"prefix":"10.1111","volume":"120","author":[{"given":"Joanna","family":"Mikulak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Negrini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrijana","family":"Klajn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosalba","family":"D\u2019Alessandro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Domenico","family":"Mavilio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacopo","family":"Meldolesi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,1,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.4161\/chan.4.6.12868"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(84)90316-6"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200003)30:3<938::AID-IMMU938>3.0.CO;2-Q"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2005.08.015"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074192"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E04-07-0577"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05259.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105156200"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/11.1.1"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.12.1953"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1791"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200408051"},{"key":"e_1_2_7_14_1","first-page":"257","article-title":"Binding of the transcription factor Slug to the L1CAM promoter is essential for transforming TGF\u2010\u03b2\u2010induced L1CAM expression in human pancreatic ductal adenocarcinoma cells","volume":"38","author":"Geismann C.","year":"2011","journal-title":"Int. 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