{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T18:29:15Z","timestamp":1774463355651,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,3,14]],"date-time":"2012-03-14T00:00:00Z","timestamp":1331683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:sec><jats:label\/><jats:p><jats:italic>J. Neurochem.<\/jats:italic> (2012) <jats:bold>121<\/jats:bold>, 362\u2013372.<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>The signaling transduction processes involved in avulsion\u2010induced motoneuron (MN) death have not been elucidated. Using the brachial plexus root avulsion rat model, we showed that avulsion\u2010activated phosphorylation of phospholipase\u2010C\u03b3 (PLC\u03b3) and protein kinase C (PKC) occurred in injured spinal MNs within 72\u2003h of injury. Moreover, some MNs positive for PLC\u03b3 and PKC are also positive for avulsion\u2010induced neuronal nitric oxide synthase (nNOS). Inhibition of PLC\u03b3\/PKC signal pathway, either with PLC\u03b3 inhibitor, 1\u2010[6\u2010((17\u03b2\u20103\u2010methoxyestra\u20101,3,5(10)\u2010trien\u201017\u2010yl) amino)hexyl]\u20101H\u2010pyrrole\u20102,5\u2010dione, or with PLC\u03b3 siRNA augmented avulsion\u2010induced MN death. 1\u2010[6\u2010((17\u03b2\u20103\u2010Methoxyestra\u20101,3,5(10)\u2010trien\u201017\u2010yl) amino)hexyl]\u20101H\u2010pyrrole\u20102,5\u2010dione also inhibited PKC phosphorylation and exacerbated avulsion\u2010induced reductions in the nNOS protein level in injured spinal segments. Moreover, activation of PLC\u03b3\/PKC signal pathway with PKC activator, phorbol\u201012\u2010myristate\u201013\u2010acetate, decreased avulsion\u2010induced MN death. The temporal profile of PLC\u03b3\/PKC signaling appears to be crucial for the survival of spinal MNs after root avulsion. Our data suggest that PLC\u03b3 mediates, while PKC and nNOS are associated with, the avulsion\u2010induced MN death in brachial plexus root avulsion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1471-4159.2012.07696.x","type":"journal-article","created":{"date-parts":[[2012,2,16]],"date-time":"2012-02-16T19:46:33Z","timestamp":1329421593000},"page":"362-372","source":"Crossref","is-referenced-by-count":11,"title":["Activation of phospholipase\u2010C\u03b3 and protein kinase C signal pathways helps the survival of spinal motoneurons injured by root 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