{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T19:01:12Z","timestamp":1762542072061},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T00:00:00Z","timestamp":1340755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:sec><jats:label \/><jats:p><jats:italic>J. Neurochem.<\/jats:italic> (2012) <jats:bold>122<\/jats:bold>, 691\u2013701.<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>The effect of icilin, a potent agonist of transient receptor potential ankyrin 1 (TRPA1) and TRPM8, on glycinergic transmission was examined in mechanically isolated rat medullary dorsal horn neurons by use of the conventional whole\u2010cell patch\u2010clamp technique. Icilin increased the frequency of glycinergic spontaneous miniature inhibitory post\u2010synaptic currents (mIPSCs) in a dose\u2010dependent manner. Either allyl isothiocyanate(AITC) or cinnamaldehyde, other TRPA1 agonists, also increased mIPSC frequency, but the extent of facilitation induced by AITC or cinnamaldehyde was less than that induced by icilin. However, menthol, a TRPM8 agonist, had no facilitatory effect on glycinergic mIPSCs. The icilin\u2010induced increase in mIPSC frequency was significantly inhibited by either HC030031, a selective TRPA1 antagonist, or ruthenium red, a non\u2010selective transient receptor potential channel blocker. Icilin failed to increase glycinergic mIPSC frequency in the absence of extracellular Ca<jats:sup>2+<\/jats:sup>, suggesting that the icilin\u2010induced increase in mIPSC frequency is mediated by the Ca<jats:sup>2+<\/jats:sup> influx from the extracellular space. In contrast, icilin still increased mIPSC frequency either in the Na<jats:sup>+<\/jats:sup>\u2010free external solution or in the presence of Cd<jats:sup>2+<\/jats:sup>, a general voltage\u2010dependent Ca<jats:sup>2+<\/jats:sup> channel blocker. The present results suggest that icilin acts on pre\u2010synaptic TRPA1\u2010like ion channels, which are permeable to Ca<jats:sup>2+<\/jats:sup>, to enhance glycinergic transmission onto medullary dorsal horn neurons. The TRPA1\u2010like channel\u2010mediated enhancement of glycinergic transmission in medullary dorsal horn neurons would contribute to the regulation of pain information from the peripheral tissues.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1471-4159.2012.07817.x","type":"journal-article","created":{"date-parts":[[2012,6,6]],"date-time":"2012-06-06T17:27:10Z","timestamp":1339003630000},"page":"691-701","source":"Crossref","is-referenced-by-count":13,"title":["TRPA1\u2010like channels enhance glycinergic transmission in medullary dorsal horn neurons"],"prefix":"10.1111","volume":"122","author":[{"given":"Jin\u2010Hwa","family":"Cho","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moon\u2010Young","family":"Jeong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In\u2010Sun","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heon\u2010Jin","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Il\u2010Sung","family":"Jang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,6,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(02)00010-X"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(92)90256-2"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2010.06833.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(04)00150-3"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm100062h"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.02.023"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.04945.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2010.06870.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.4.6"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19960101)364:1<169::AID-CNE13>3.0.CO;2-U"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-4-30"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00400.2001"},{"key":"e_1_2_8_14_1","first-page":"349","article-title":"Transient receptor potential A1 is involved in cold\u2010induced contraction in the isolated rat colon smooth muscle","volume":"62","author":"Dong Y.","year":"2010","journal-title":"Sheng Li Xue Bao"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-4-48"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neures.2003.12.005"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00511.2009"},{"key":"e_1_2_8_18_1","doi-asserted-by":"crossref","first-page":"1646","DOI":"10.1523\/JNEUROSCI.14-03-01646.1994","article-title":"\u03bc\u2010Opioid agonists inhibit spinal trigeminal substantia gelatinosa neurons in guinea pig and rat","volume":"14","author":"Grudt T. 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