{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:38:07Z","timestamp":1791279487879,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,8,20]],"date-time":"2012-08-20T00:00:00Z","timestamp":1345420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:sec><jats:label\/><jats:p><jats:italic>J. Neurochem<\/jats:italic>. (2012)<jats:bold>122<\/jats:bold>, 1010\u20131022.<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Amyloid precursor protein (APP) is involved in the pathogenesis of Alzheimer\u2019s disease. It is axonally transported, endocytosed and sorted to different cellular compartments where amyloid beta (A\u03b2) is produced. However, the mechanism of APP trafficking remains unclear. We present evidence that huntingtin associated protein 1 (HAP1) may reduce A\u03b2 production by regulating APP trafficking to the non\u2010amyloidogenic pathway. HAP1 and APP are highly colocalized in a number of brain regions, with similar distribution patterns in both mouse and human brains. They are associated with each other, the interacting site is the 371\u2013599 of HAP1. APP is more retained in cis\u2010Golgi, trans\u2010Golgi complex, early endosome and ER\u2010Golgi intermediate compartment in HAP1\u2212\/\u2212 neurons. HAP1 deletion significantly alters APP endocytosis and reduces the re\u2010insertion of APP into the cytoplasmic membrane. Amyloid precursor protein\u2010YFP(APP\u2010YFP) vesicles in HAP1\u2212\/\u2212 neurons reveal a decreased trafficking rate and an increased number of motionless vesicles. Knock\u2010down of HAP1 protein in cultured cortical neurons of Alzheimer\u2019s disease mouse model increases A\u03b2 levels. Our data suggest that HAP1 regulates APP subcellular trafficking to the non\u2010amyloidogenic pathway and may negatively regulate A\u03b2 production in neurons.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1471-4159.2012.07845.x","type":"journal-article","created":{"date-parts":[[2012,6,25]],"date-time":"2012-06-25T19:31:49Z","timestamp":1340652709000},"page":"1010-1022","source":"Crossref","is-referenced-by-count":35,"title":["Huntingtin associated protein 1 regulates trafficking of the amyloid precursor protein and modulates amyloid beta levels in neurons"],"prefix":"10.1111","volume":"122","author":[{"given":"Gui\u2010Zhi","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Jun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting\u2010Hua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Guo","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Hua","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Fu","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,8,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)85352-8"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503689102"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2290-06.2006"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309561200"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023007"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/6.13.2205"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M303347200"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/349704a0"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90312-E"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(91)90609-V"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1566067"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411296200"},{"key":"e_1_2_8_14_1","first-page":"562","article-title":"Preparation of dissociated mouse cortical neuron cultures","volume":"10","author":"Hilgenberg L. 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