{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T19:31:38Z","timestamp":1789673498108,"version":"4.0.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,8,14]],"date-time":"2012-08-14T00:00:00Z","timestamp":1344902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Disruption of neuronal Ca\n                    <jats:sup>2+<\/jats:sup>\n                    homeostasis plays a well\u2010established role in cell death in a number of neurodegenerative disorders. Recent evidence suggests that proteolysis of the type 1 inositol 1,4,5\u2010trisphosphate receptor (InsP\n                    <jats:sub>3<\/jats:sub>\n                    R1), a Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release channel on the endoplasmic reticulum, generates a dysregulated channel, which may contribute to aberrant Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling and neurodegeneration in disease states. However, the specific effects of InsP\n                    <jats:sub>3<\/jats:sub>\n                    R1 proteolysis on neuronal Ca\n                    <jats:sup>2+<\/jats:sup>\n                    homeostasis are unknown, as are the functional contributions of this pathway to neuronal death. This study evaluates the consequences of calpain\u2010mediated InsP\n                    <jats:sub>3<\/jats:sub>\n                    R1 proteolysis on neuronal Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling and survival using adeno\u2010associated viruses to express a recombinant cleaved form of the channel (capn\u2010InsP\n                    <jats:sub>3<\/jats:sub>\n                    R1) in rat primary cortical neurons. Here, we demonstrate that expression of capn\u2010InsP\n                    <jats:sub>3<\/jats:sub>\n                    R1 in cortical cultures reduced cellular viability. This effect was associated with increased resting cytoplasmic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration ([Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    ), increased [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    response to glutamate, and enhanced sensitivity to excitotoxic stimuli. Together, our results demonstrate that InsP\n                    <jats:sub>3<\/jats:sub>\n                    R1 proteolysis disrupts neuronal Ca\n                    <jats:sup>2+<\/jats:sup>\n                    homeostasis, and potentially acts as a feed\u2010forward pathway to initiate or execute neuronal death.\n                  <\/jats:p>","DOI":"10.1111\/j.1471-4159.2012.07859.x","type":"journal-article","created":{"date-parts":[[2012,7,5]],"date-time":"2012-07-05T02:06:04Z","timestamp":1341453964000},"page":"147-158","source":"Crossref","is-referenced-by-count":25,"title":["Calpain\u2010cleaved type 1 inositol 1,4,5\u2010trisphosphate receptor impairs ER Ca\n                    <sup>2+<\/sup>\n                    buffering and causes neurodegeneration in primary cortical neurons"],"prefix":"10.1111","volume":"123","author":[{"given":"Catherine M.","family":"Kopil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam P.","family":"Siebert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Kevin Foskett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Neumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,8,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050949"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M403872200"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2005.12.008"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/35036035"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1155"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05860.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M509966200"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M001724200"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C100094200"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.06.007"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2008.04.015"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.37.1.205"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-17-05312.1996"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1996.7.17-2101"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(99)01492-7"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0397-306"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-010-0826-0"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00035.2006"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(00)76339-9"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00029.2002"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.48.34433"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neures.2005.02.004"},{"key":"e_1_2_9_24_1","doi-asserted-by":"crossref","first-page":"6477","DOI":"10.1016\/S0021-9258(18)53276-2","article-title":"Detergent solubility of the inositol trisphosphate receptor in rat brain membranes. Evidence for association of the receptor with ankyrin","volume":"268","author":"Joseph S. K.","year":"1993","journal-title":"J. Biol. Chem."},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.254177"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-10-06834.1995"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200308809"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7401087"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2007.00275.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(00)01548-4"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(97)00055-5"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.12-12-04834.1992"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20030599"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4617-05.2006"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1150"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.11.015"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-07-03051.1993"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M807288200"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.110.003814"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2312"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1042\/BC20070086"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2003.tb00238.x"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.104.056002"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1302"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1471-4159.2012.07859.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1471-4159.2012.07859.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T11:58:53Z","timestamp":1695470333000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1471-4159.2012.07859.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,14]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,10]]}},"alternative-id":["10.1111\/j.1471-4159.2012.07859.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1471-4159.2012.07859.x","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,14]]}}}