{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T21:07:52Z","timestamp":1791061672598,"version":"4.1.0"},"reference-count":59,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,8,3]],"date-time":"2012-08-03T00:00:00Z","timestamp":1343952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:sec><jats:label\/><jats:p><jats:italic>J. Neurochem.<\/jats:italic> (2012) <jats:bold>122<\/jats:bold>, 1081\u20131091.<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Dual\u2010specificity tyrosine(Y)\u2010phosphorylation\u2010regulated kinase 1A (Dyrk1A) is a protein kinase that might be responsible for mental retardation and early onset of Alzheimer\u2019s disease in Down\u2019s syndrome patients. Dyrk1A plays a role in many cellular pathways through phosphorylation of diverse substrate proteins; however, its role in synaptic vesicle exocytosis is poorly understood. Munc18\u20101, a central regulator of neurotransmitter release, interacts with Syntaxin 1 and X11\u03b1. Syntaxin 1 is a key soluble N\u2010ethylmaleimide\u2010sensitive factor attachment protein receptor protein involved in synaptic vesicle docking\/fusion events, and X11\u03b1 modulates amyloid precursor protein processing and \u03b2 amyloid generation. In this study, we demonstrate that Dyrk1A interacts with and phosphorylates Munc18\u20101 at the Thr<jats:sup>479<\/jats:sup> residue. The phosphorylation of Munc18\u20101 at Thr<jats:sup>479<\/jats:sup> by Dyrk1A stimulated binding of Munc18\u20101 to Syntaxin 1 and X11\u03b1. Furthermore, the levels of phospho\u2010Thr<jats:sup>479<\/jats:sup>\u2010Munc18\u20101 were enhanced in the brains of transgenic mice over\u2010expressing Dyrk1A protein, providing <jats:italic>in vivo<\/jats:italic> evidence of Munc18\u20101 phosphorylation by Dyrk1A. These results reveal a link between Munc18\u20101 and Dyrk1A in synaptic vesicle trafficking and amyloid precursor protein processing, suggesting that up\u2010regulated Dyrk1A in Down\u2019s syndrome and Alzheimer\u2019s disease brains may contribute to some pathological features, including synaptic dysfunction and cognitive defect through abnormal phosphorylation of Munc18\u20101.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1471-4159.2012.07861.x","type":"journal-article","created":{"date-parts":[[2012,7,6]],"date-time":"2012-07-06T01:56:58Z","timestamp":1341539818000},"page":"1081-1091","source":"Crossref","is-referenced-by-count":22,"title":["Phosphorylation of Munc18\u20101 by Dyrk1A regulates its interaction with Syntaxin 1 and X11\u03b1"],"prefix":"10.1111","volume":"122","author":[{"given":"Jung\u2010Hwa","family":"Park","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min\u2010Su","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeun\u2010Soo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woo\u2010Joo","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sul\u2010Hee","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,8,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2010.12.024"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.10.169"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2005.12.006"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.18.1915"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E11-02-0101"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e07-07-0662"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211114200"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.24.14761"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-010-9591-z"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111101200"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-78887-6_4"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0611318104"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2005.03.020"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.7.4027"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.18.6636-6647.2002"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.13.7265"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5210-09.2010"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/5.9.1305"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1999.5775"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e09-08-0712"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/366347a0"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201823200"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.252774899"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2855-06.2006"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2481-08.2008"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.253971"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.7.3488"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3655-07.2007"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000321994"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddl437"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2008.03.038"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2004.10.012"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312711200"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.020230"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/35006120"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M513497200"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.50.31459"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2007.07.007"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-009-0123-2"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M700227200"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e08-05-0538"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M707358200"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.05075.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2010.06769.x"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801353200"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200804075"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M609312200"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.12.016"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.1135"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.9.4957"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0597-28"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-010-9581-1"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1996.0636"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1161748"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.93.41"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2010.07954.x"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5454.864"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M414353200"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9021878"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1471-4159.2012.07861.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1471-4159.2012.07861.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T05:24:16Z","timestamp":1698643456000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1471-4159.2012.07861.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,3]]},"references-count":59,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["10.1111\/j.1471-4159.2012.07861.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1471-4159.2012.07861.x","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,3]]}}}