{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T17:53:20Z","timestamp":1758477200695},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,4,12]],"date-time":"2006-04-12T00:00:00Z","timestamp":1144800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Fundamemntal Clinical Pharma"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although anaesthetics are widely used to alleviate stress in endotoxaemic animals, these drugs themselves may interfere with the effects of lipopolysaccharide (LPS). The effects of LPS on serum glucose, biochemical markers of hepatic, renal and pancreatic exocrine function, and lung myeloperoxidase (MPO) activity were compared using anaesthesia with either urethane\/chloralose or pentobarbitone. Groups of 10\u201313 of C57B1\/6 mice (22.3\u2003\u00b1\u20030.18\u2003g) were treated with 40\u2003mg\/kg LPS or the same volume of saline (10\u2003mL\/kg, i.p.) at time 0, Animals were anaesthetized either with urethane (1000\u2003mg\/kg) and chloralose (50\u2003mg\/kg) or with pentobarbitone (90\u2003mg\/kg, i.p.) after 2\u2003h and blood and lung samples obtained after 6\u2003h. In pentobarbitone\u2010anaesthetized mice, LPS caused hypoglycaemia and increased serum levels of alanine aminotransferase (ALT), lipase and creatinine suggesting damage\/dysfunction of liver, exocrine pancreas and kidney respectively. Lung tissue MPO activity, an indicator of neutrophil infiltration, was also increased. Urethane\/chloralose\u2010treated mice demonstrated hypoglycaemia and enhanced serum levels of ALT and creatinine in response to LPS, but failed to show LPS\u2010induced increases in serum lipase and lung MPO activity. It is concluded that while pentobarbitone may be successfully used in experimental models of endotoxaemia in mice, anaesthesia with urethane and chloralose may protect mice against LPS\u2010mediated damage\/dysfunction in the exocrine pancreas and in the lung, and therefore, is not recommended in studies on endotoxaemic mice.<\/jats:p>","DOI":"10.1111\/j.1472-8206.2006.00413.x","type":"journal-article","created":{"date-parts":[[2006,4,23]],"date-time":"2006-04-23T16:20:31Z","timestamp":1145809231000},"page":"379-384","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of urethane\/chloralose and pentobarbitone anaesthesia for examining effects of bacterial lipopolysaccharide in mice"],"prefix":"10.1111","volume":"20","author":[{"given":"Hamid R.","family":"Kazerani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian L.","family":"Furman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,4,25]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00338.2002"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1152\/jappl.1996.81.5.2304","article-title":"Urethane anaesthesia protects rats against lethal endotoxemia and reduces TNF\u2010alpha release","volume":"81","author":"Kotanidou A.","year":"1996","journal-title":"J. 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