{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T05:25:20Z","timestamp":1697001920875},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,6,14]],"date-time":"2005-06-14T00:00:00Z","timestamp":1118707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Auton Autocoid Pharmacol"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:bold>1<\/jats:bold>\u2003Experiments were carried out to characterize the possible adrenergic properties of the 5\u2010HT<jats:sub>1A<\/jats:sub> antagonists WAY 100635 and MM\u201077 using the mouse isolated vasa deferentia preparation.<\/jats:p><jats:p> <jats:bold>2<\/jats:bold>\u2003When vasa deferentia were preincubated for 10\u2003min in the presence of MM\u201077 (10<jats:sup>\u22128<\/jats:sup>\u201310<jats:sup>\u22126<\/jats:sup>\u2003<jats:sc>m<\/jats:sc>) or WAY100635 (10<jats:sup>\u22128<\/jats:sup>\u20137\u2003\u00d7\u200310<jats:sup>\u22127<\/jats:sup>\u2003<jats:sc>m<\/jats:sc>), a concentration\u2010dependent inhibition of the contractile response to submaximal electrical field stimulation (10\u2003Hz, 50\u2003V, 50\u2003ms) was observed with pIC<jats:sub>50<\/jats:sub> values of 7.05\u2003\u00b1\u20030.01 and 6.85\u2003\u00b1\u20030.1 respectively.<\/jats:p><jats:p> <jats:bold>3<\/jats:bold>\u2003MM\u201077 (10<jats:sup>\u22128<\/jats:sup>\u201310<jats:sup>\u22126<\/jats:sup>\u2003<jats:sc>m<\/jats:sc>) antagonized the contractile responses of the vasa deferentia to phenylephrine (PE) (10<jats:sup>\u22126<\/jats:sup>\u201310<jats:sup>\u22123<\/jats:sup>\u2003<jats:sc>m<\/jats:sc>) in a concentration\u2010dependent manner. Schild plots of these data were linear and yielded a mean <jats:italic>\u03c1<\/jats:italic><jats:italic>A<\/jats:italic><jats:sub>2<\/jats:sub> value of 6.81\u2003\u00b1\u20030.084. The mean slope was 1.42\u2003\u00b1\u20030.22.<\/jats:p><jats:p> <jats:bold>4<\/jats:bold>\u2003WAY100635 (10<jats:sup>\u22128<\/jats:sup>\u201310<jats:sup>\u22126<\/jats:sup>\u2003<jats:sc>m<\/jats:sc>) antagonized the contractile responses of the vasa deferentia to PE (10<jats:sup>\u22126<\/jats:sup>\u201310<jats:sup>\u22123<\/jats:sup>\u2003<jats:sc>m<\/jats:sc>) in a concentration\u2010dependent manner. Schild plots of these data were linear and yielded a mean <jats:italic>\u03c1<\/jats:italic><jats:italic>A<\/jats:italic><jats:sub>2<\/jats:sub> value of 7.05\u2003\u00b1\u20030.08. The mean slope was 0.97\u2003\u00b1\u20030.1.<\/jats:p><jats:p> <jats:bold>5<\/jats:bold>\u2003The results suggest that while WAY100635 acts as a competitive antagonist at <jats:italic>\u03b1<\/jats:italic><jats:sub>1<\/jats:sub>\u2010adrenoceptors, MM\u201077 displays non\u2010competitive antagonist characteristics at this receptor subtype.<\/jats:p><jats:p> <jats:bold>6<\/jats:bold>\u2003These results may have important implications for the use of these compounds as 5\u2010HT<jats:sub>1A<\/jats:sub> receptor antagonists in <jats:italic>in vivo<\/jats:italic> studies.<\/jats:p>","DOI":"10.1111\/j.1474-8673.2005.00341.x","type":"journal-article","created":{"date-parts":[[2005,6,14]],"date-time":"2005-06-14T10:56:50Z","timestamp":1118746610000},"page":"121-128","source":"Crossref","is-referenced-by-count":3,"title":["A comparison of the effects of the 5HT<sub>1A<\/sub> antagonists MM\u201077 and WAY\u2010100635 on the mouse isolated vasa deferentia"],"prefix":"10.1111","volume":"25","author":[{"given":"M. J.","family":"Arkle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Arkle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. S.","family":"Ebenezer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-3623(01)00073-8"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(00)00798-6"},{"key":"e_1_2_5_4_1","article-title":"Effects of the putative 5HT1A agonist MM\u201077 on feeding in rats","author":"ARKLE M.J.","year":"2004","journal-title":"Proc. Br. Pharmacol. Soc."},{"key":"e_1_2_5_5_1","article-title":"A comparison of the effects of the putative 5HT1A antagonist MM\u201077 with WAY\u2010100635 on the mouse isolated vasa deferentia","author":"ARKLE M.J.","year":"2004","journal-title":"Proc. Br. Pharmacol. Soc."},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1959.tb00928.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-8673.1991.tb00322.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(93)90195-N"},{"key":"e_1_2_5_9_1","first-page":"216","article-title":"Buspirone effectiveness, safety and mechanisms of action","volume":"8","author":"COHN J.B.","year":"1987","journal-title":"Intern. Med. Spec."},{"key":"e_1_2_5_10_1","volume-title":"The Biochemical Basis of Neuropharmacology","author":"COOPER J.R.","year":"1991"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(85)90005-X"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199201000-00016"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199211000-00019"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9384(99)00050-5"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9384(01)00467-X"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(91)90250-T"},{"key":"e_1_2_5_17_1","first-page":"91P","article-title":"A pharmacological profile of WAY\u2010100635, a potent and selective 5\u2010HT1A receptor antagonist","volume":"112","author":"FLETCHER A.","year":"1994","journal-title":"Br. J. Pharmacol."},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-4328(96)00118-0"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(95)00234-C"},{"key":"e_1_2_5_20_1","first-page":"283","volume-title":"Catecholamines, Handbook of Experimental Pharmacology","author":"FURCHGOTT R.F.","year":"1972"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(88)90410-4"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00165749"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(75)90879-3"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00179315"},{"key":"e_1_2_5_25_1","first-page":"165","article-title":"The classification of drugs and drug receptors in isolated tissues","volume":"36","author":"KENAKIN T.P.","year":"1984","journal-title":"Pharmacol. Rev."},{"key":"e_1_2_5_26_1","volume-title":"Textbook of In Vitro Practical Pharmacology","author":"KITCHEN I.","year":"1984"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm00043a014"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm950662c"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0896(98)00238-7"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0969-8051(96)00061-3"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-894X(98)00451-X"},{"key":"e_1_2_5_32_1","first-page":"106","article-title":"Dopamine agonist activity of 8\u2010OH\u2010DPAT","volume":"306","author":"SMITH C.F.","year":"1990","journal-title":"Arch. Int. Pharmacodyn. Ther."},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890010103"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-8673.1991.tb00321.x"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm00097a002"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1474-8673.2002.00257.x"}],"container-title":["Autonomic and Autacoid Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1474-8673.2005.00341.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1474-8673.2005.00341.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T17:52:09Z","timestamp":1696960329000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1474-8673.2005.00341.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,6,14]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,7]]}},"alternative-id":["10.1111\/j.1474-8673.2005.00341.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1474-8673.2005.00341.x","archive":["Portico"],"relation":{},"ISSN":["1474-8665","1474-8673"],"issn-type":[{"value":"1474-8665","type":"print"},{"value":"1474-8673","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,6,14]]}}}