{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T20:00:49Z","timestamp":1778702449079,"version":"3.51.4"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,6,19]],"date-time":"2006-06-19T00:00:00Z","timestamp":1150675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Auton Autocoid Pharmacol"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>1<\/jats:bold>\u2003In the bovine ciliary muscle, stimulation of muscarinic receptors with carbachol (CCh) opens two types of non\u2010selective cation channels (NSCCS and NSCCL) with widely different unitary conductances (100\u2003fS and 35\u2003pS). Here we examined the dependence of the activity of NSCCS on the agonist (CCh) concentration by whole\u2010cell voltage clamp in freshly isolated bovine ciliary muscle cells. We also examined the sensitivity of CCh\u2010evoked NSCCS currents to several muscarinic receptor antagonists.<\/jats:p><jats:p><jats:bold>2<\/jats:bold>\u2003The voltage clamp experiments were carried out using Ba<jats:sup>2+<\/jats:sup>as the charge carrier, as this divalent cation is the most permeant for NSCCS of the alkali and alkaline earth metal ions hitherto examined, whereas it is relatively impermeant to NSCCL. For the dose\u2013activation relationship obtained, the apparent dissociation constant<jats:italic>K<\/jats:italic>was estimated to be 0.5\u2003\u00b1\u20030.2\u2003<jats:italic>\u03bc<\/jats:italic><jats:sc>m<\/jats:sc>(<jats:italic>n<\/jats:italic>\u2003=\u200331), a value of an order of magnitude smaller than the one reported for CCh\u2010evoked NSCCL currents in our previous experiments.<\/jats:p><jats:p><jats:bold>3<\/jats:bold>\u2003In the dose\u2010inhibition experiments we observed that the CCh\u2010evoked NSCCS currents were inhibited by the muscarinic antagonists with the following potency sequence: atropine\u2003\u2248\u20034\u2010DAMP\u2003&gt;&gt;\u2003pirenzepine\u2003&gt;\u2003AF\u2010DX116, indicating that the activation of NSCCS by CCh is mediated by an M<jats:sub>3<\/jats:sub>muscarinic receptor.<\/jats:p><jats:p><jats:bold>4<\/jats:bold>\u2003We have previously shown by reverse transcriptase\u2010polymerase chain reaction that the bovine ciliary muscle contains mRNAs for several transient receptor potential channel homologues (TRPC1, TRPC3, TRPC4 and TRPC6) which are attracting attention as molecular candidates for receptor\u2010operated NSCCs. In the present experiments, we succeeded in visually identifying these TRPCs in the plasma membrane of cultured bovine ciliary muscle cells by immunofluorescence microscopy.<\/jats:p>","DOI":"10.1111\/j.1474-8673.2006.00347.x","type":"journal-article","created":{"date-parts":[[2006,6,19]],"date-time":"2006-06-19T10:08:50Z","timestamp":1150711730000},"page":"285-292","source":"Crossref","is-referenced-by-count":9,"title":["Agonist and antagonist sensitivity of non\u2010selective cation channel currents evoked by muscarinic receptor stimulation in bovine ciliary muscle cells"],"prefix":"10.1111","volume":"26","author":[{"given":"R.","family":"Sugawara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Takai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Miyazu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Ohinata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Takai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,6,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1979.59.3.606"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(89)90054-4"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-2873-3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-711X(94)90178-3"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80437-7"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002705200"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704468"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1980.sp013426"},{"key":"e_1_2_7_10_1","volume-title":"Statistical Methods","author":"SNEDECOR G.W.","year":"1980"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00240-9"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1983.tb08819.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050335"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.065607"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1834247100"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1016\/S0022-3565(24)38322-3","article-title":"Effects of specific modifications of several hydroxyls of tetrodotoxin on its affinity to rat brain membrane","volume":"283","author":"YOTSU\u2010YAMASHITA M.","year":"1999","journal-title":"J. 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