{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:41:34Z","timestamp":1762270894871},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,3,20]],"date-time":"2006-03-20T00:00:00Z","timestamp":1142812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Auton Autocoid Pharmacol"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:bold>1<\/jats:bold>\u20035\u2010Hydroxytryptamine (5\u2010HT) receptor subtypes involved in 5\u2010HT\u2010induced contraction of the chicken gastrointestinal tract were characterized pharmacologically using subtype\u2010selective agonists and antagonists. The proventriculus (area of stomach adjacent to the oesophagus) and ileum are examined.<\/jats:p><jats:p> <jats:bold>2<\/jats:bold>\u20035\u2010HT applied cumulatively caused sustained contraction of the proventriculus that was not decreased by tetrodotoxin, atropine or <jats:sc>l<\/jats:sc>\u2010nitro\u2010arginine methylester (<jats:sc>l<\/jats:sc>\u2010NAME). <jats:italic>\u03b1<\/jats:italic>\u2010Methyl\u20105\u2010HT showed the same potency as that of 5\u2010HT, indicating the involvement of the 5\u2010HT<jats:sub>2<\/jats:sub> receptor. (\u00b1)\u20101\u2010(2,5\u2010dimethoxy\u20104\u2010iodophenyl)\u20102\u2010amino\u2010propane (DOI), 5\u2010methoxytryptamine and 1\u2010(3\u2010chlorophenyl)piperazine hydrochloride (mCPP) were potent, and 2\u2010methyl\u20105\u2010HT, 5\u2010carboxamidotryptamine, BW723C86 and 6\u2010chloro\u20102\u2010(1\u2010piperazinyl)pyrazine hydrochloride (MK212) were moderate, but (\u00b1)\u20108\u2010hydroxy\u20102\u2010dipropylaminotetralin hydrobromide (8\u2010OH\u2010DPAT), [endo\u2010<jats:italic>N<\/jats:italic>\u20108\u2010methyl\u20108\u2010azabicyclo\u2010(3,2,1)oct\u20103\u2010yl]\u20102,3\u2010dihydro\u2010(1\u2010methyl)ethyl\u20102\u2010oxo\u20101H\u2010benzimidazol\u20101\u2010carboxamide (BIMU\u20108) and cisapride were weak agonists. Correlation of pEC<jats:sub>50<\/jats:sub> values of these agonists with documented pEC<jats:sub>50<\/jats:sub> values for 5\u2010HT<jats:sub>2C<\/jats:sub> receptor was higher than 5\u2010HT<jats:sub>2A<\/jats:sub> and 5\u2010HT<jats:sub>2B<\/jats:sub>.<\/jats:p><jats:p> <jats:bold>3<\/jats:bold>\u2003Cinanserin, ketanserin, methiothepin, methysergide, mianserin, (8\u2010[5\u2010(2,4\u2010dimethoxy\u20105\u2010(4\u2010trifluoromethylphenylsulphonamido)phenyl\u20105\u2010oxopentyl)\u20101,3,8\u2010triazaspiro[4,5]decane\u20102,4\u2010dione hydrochloride (RS102221), <jats:italic>N<\/jats:italic>\u2010(1\u2010methyl\u20101H\u2010indolyl\u20105\u2010yl)\u2010<jats:italic>N<\/jats:italic>\u2032\u2010(3\u2010methyl\u20105\u2010isothiazolyl)urea (SB204741), spiperone and <jats:italic>N<\/jats:italic>\u2010desmethylclozapine concentration\u2010dependently inhibited the contractile responses to 5\u2010HT. Correlation of p<jats:italic>K<\/jats:italic><jats:sub>b<\/jats:sub>\/p<jats:italic>A<\/jats:italic><jats:sub>2<\/jats:sub> of antagonists with documented p<jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> for 5\u2010HT<jats:sub>2C<\/jats:sub> receptor was highest among 5\u2010HT<jats:sub>2<\/jats:sub> receptor subtypes.<\/jats:p><jats:p> <jats:bold>4<\/jats:bold> In the methysergide\u2010 and ketanserin\u2010treated proventriculus, 5\u2010HT, 2\u2010methyl\u20105\u2010HT and cisapride did not enhance the electrical field stimulation (5\u2003Hz)\u2010induced cholinergic contractions.<\/jats:p><jats:p> <jats:bold>5<\/jats:bold>\u20035\u2010HT applied non\u2010cumulatively caused transient contraction of ileum, and the responses were partly decreased by atropine or tetrodotoxin. 5\u2010Methoxytryptamine, <jats:italic>\u03b1<\/jats:italic>\u2010methyl\u20105\u2010HT, 5\u2010carboxamidotryptamine, L692,247 and DOI were potent agonists. However, 2\u2010methyl\u20105\u2010HT, cisapride, BW723C86, 8\u2010OH\u2010DPAT and 5\u2010nonyloxytryptamine, mCPP and MK212 were less effective. Ketanserin and methysergide decreased the 5\u2010HT\u2010induced ileal contraction, but neither GR113808 nor SB269970 inhibited the responses.<\/jats:p><jats:p> <jats:bold>6<\/jats:bold>\u2003In conclusion, 5\u2010HT causes contraction of the proventriculus via 5\u2010HT<jats:sub>2C<\/jats:sub>\u2010like receptors present on smooth muscle. 5\u2010HT also causes contraction of the ileum, but the underlying mechanisms are complex, involving neural and smooth muscle components, and both 5\u2010HT<jats:sub>1<\/jats:sub>\u2010 and 5\u2010HT<jats:sub>2<\/jats:sub>\u2010like receptors. Neural 5\u2010HT receptors similar to 5\u2010HT<jats:sub>3<\/jats:sub>\/5\u2010HT<jats:sub>4<\/jats:sub> receptors were not found in the chicken proventriculus and ileum.<\/jats:p>","DOI":"10.1111\/j.1474-8673.2006.00365.x","type":"journal-article","created":{"date-parts":[[2006,3,20]],"date-time":"2006-03-20T13:31:34Z","timestamp":1142861494000},"page":"157-168","source":"Crossref","is-referenced-by-count":17,"title":["Pharmacological characterization of 5\u2010hydroxytryptamine\u2010induced contraction in the chicken gastrointestinal tract"],"prefix":"10.1111","volume":"26","author":[{"given":"T.","family":"Kitazawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Ukai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Komori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Taneike","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,3,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(96)00777-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1959.tb00928.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1994.tb13072.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-6147(00)88991-9"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13941.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(94)90059-0"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(97)00049-X"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902580409"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(96)00297-X"},{"key":"e_1_2_6_11_1","first-page":"121","article-title":"Interactions of serotonin with multiple receptors and neurotransmitters in the guinea\u2010pig isolated colon","volume":"329","author":"BRIEJER M.R.","year":"1995","journal-title":"Arch. 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