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Neonatal human skin fibroblast cultures were stimulated with carbachol in presence or absence of specific antagonists and the following parameters were measured: identification of mAChR subtypes, DNA synthesis, inositol phosphates (InsP) production and CD40 expression.<\/jats:p><jats:p> <jats:bold>2<\/jats:bold>\u2003Human fibroblasts express mAChR with <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> 0.47\u2003\u00b1\u20030.11\u2003n<jats:sc>m<\/jats:sc> and <jats:italic>B<\/jats:italic><jats:sub>max<\/jats:sub> 236\u2003\u00b1\u200322\u2003fmol\u2003mg\u2003protein<jats:sup>\u22121<\/jats:sup>. Carbachol stimulates DNA synthesis, InsP and the expression of CD40. All these effects were inhibited by atropine, mustard hydrochloride (4\u2010DAMP) and pirenzepine but not by AF\u2010DX 116 and tropicamide, indicating that M<jats:sub>3<\/jats:sub> and M<jats:sub>1<\/jats:sub> mAChR are implicated in carbachol action. The relative <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> of the antagonists obtained by competition binding assay was in parallel to the relative potency for blocking both carbachol\u2010stimulated InsP accumulation and DNA synthesis.<\/jats:p><jats:p> <jats:bold>3<\/jats:bold>\u2003The intracellular pathway leading to carbachol\u2010induced biological effects involved phospholipase C and calcium\/calmodulin, as U\u201073122 and trifluoroperazine blocked carbachol effects, respectively. Calphostin C, a protein kinase C inhibitor, had no effect, indicating that this enzyme does not participate in the system.<\/jats:p><jats:p> <jats:bold>4<\/jats:bold>\u2003These results may contribute to a better understanding of the modulatory role of the parasympathetic muscarinic system on normal human fibroblast function.<\/jats:p>","DOI":"10.1111\/j.1474-8673.2006.00369.x","type":"journal-article","created":{"date-parts":[[2006,6,19]],"date-time":"2006-06-19T10:08:50Z","timestamp":1150711730000},"page":"293-301","source":"Crossref","is-referenced-by-count":5,"title":["Muscarinic cholinoceptor activation modulates DNA synthesis and CD40 expression in fibroblast cells"],"prefix":"10.1111","volume":"26","author":[{"given":"M.","family":"Casanova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Furl\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Sterin\u2010Borda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. 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