{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T12:14:22Z","timestamp":1763036062196},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,8,24]],"date-time":"2005-08-24T00:00:00Z","timestamp":1124841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We have examined the relationship between nucleotide excision of the main UV\u2010induced photoproduct, the cyclobutane pyrimidine dimer and<jats:italic>in vitro<\/jats:italic>cellular senescence. An<jats:italic>in situ<\/jats:italic>semiquantitative immunocytochemical assay has demonstrated that, following a UV\u2010C dose of 15\u00a0J\u00a0m<jats:sup>\u22122<\/jats:sup>, young human dermal fibroblasts maintained in a high level of serum are more efficient than senescent fibroblasts in the removal of dimers. However, in G<jats:sub>0<\/jats:sub>\u2010arrested cultures (serum\u2010starved), young fibroblasts are compromised in their ability to remove dimers and are significantly less efficient than senescent cells in this process. Supplementation of the culture medium with 0.1\u00a0m<jats:sc>m<\/jats:sc>deoxyribonucleosides enhances the removal of dimers in both young and senescent fibroblasts in proliferating or serum\u2010starved cells. These data indicate that overall there is a modest but significant reduction in nucleotide excision of dimer photoproducts in cells as they age<jats:italic>in vitro<\/jats:italic>. In addition, G<jats:sub>0<\/jats:sub>\u2010arrested young cells exhibit reduced removal of dimers, although this can be complemented by deoxyribonucleoside addition. In addition, this<jats:italic>in situ<\/jats:italic>assay has revealed heterogeneity in both susceptibility to UV\u2010C\u2010induced damage and excision. Overall, we provide evidence of reduced UV\u2010induced damage excision in senescent compared with young fibroblasts, and demonstrate modulation of these processes in young and senescent cells under specific growth conditions.<\/jats:p>","DOI":"10.1111\/j.1474-9726.2005.00167.x","type":"journal-article","created":{"date-parts":[[2005,8,24]],"date-time":"2005-08-24T15:46:36Z","timestamp":1124898396000},"page":"247-255","source":"Crossref","is-referenced-by-count":12,"title":["Heterogeneity of dimer excision in young and senescent human dermal fibroblasts"],"prefix":"10.1111","volume":"4","author":[{"given":"Jennifer","family":"Boyle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian R.","family":"Kill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher N.","family":"Parris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,8,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2004.12.002"},{"key":"e_1_2_5_3_1","first-page":"609","article-title":"Hypersensitivity of human lymphocytes to UV\u2010B and solar irradiation","volume":"53","author":"Arlett CF","year":"1993","journal-title":"Cancer Res."},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2004.02.002"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.02.003"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2000.4826"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(74)90165-1"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(73)90394-7"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.11.5.9141498"},{"key":"e_1_2_5_10_1","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1096\/fj.01-0829fje","article-title":"DNA oligonucleotide treatment corrects the age associated decline in DNA repair capacity","volume":"16","author":"Goukassian DA","year":"2002","journal-title":"FASEB J."},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.14.10.1325"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(95)00110-7"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"1854","DOI":"10.1093\/clinchem\/41.12.1854","article-title":"DNA repair and epidemiology of basal cell carcinoma","volume":"41","author":"Grossman L","year":"1995","journal-title":"Clin. Chem."},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0047-6374(76)90009-9"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(65)90211-9"},{"key":"e_1_2_5_16_1","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1093\/oso\/9780199637973.003.0011","volume-title":"Animal Cell Culture, A Practical Approach","author":"Kill IR","year":"2000"},{"key":"e_1_2_5_17_1","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1242\/jcs.107.2.571","article-title":"The expression of proliferation\u2010dependent antigens during the lifespan of normal and progeroid human fibroblasts in culture","volume":"107","author":"Kill IR","year":"1994","journal-title":"J. Cell Sci."},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1979.0083"},{"key":"e_1_2_5_19_1","doi-asserted-by":"crossref","unstructured":"LehmannAR(2003)DNA repair deficient diseases xeroderma pigmentosum Cockayne syndrome and trichothiodystrophy.Biochimie85 1101\u20131111.","DOI":"10.1016\/j.biochi.2003.09.010"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/21447"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.23.8878"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0921-8777(96)00029-8"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.2307\/3573725"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0047-6374(92)90140-9"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041170309"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001640306"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.65.070196.000355"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1095"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1568-7864(03)00006-5"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0047-6374(87)90109-6"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0047-6374(97)00108-5"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.4.1614"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0531-5565(01)00111-5"}],"container-title":["Aging Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1474-9726.2005.00167.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1474-9726.2005.00167.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T07:13:31Z","timestamp":1706512411000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1474-9726.2005.00167.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,8,24]]},"references-count":32,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2005,10]]}},"alternative-id":["10.1111\/j.1474-9726.2005.00167.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1474-9726.2005.00167.x","archive":["Portico"],"relation":{},"ISSN":["1474-9718","1474-9726"],"issn-type":[{"value":"1474-9718","type":"print"},{"value":"1474-9726","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,8,24]]}}}