{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:43:38Z","timestamp":1790642618333,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,8,30]],"date-time":"2012-08-30T00:00:00Z","timestamp":1346284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Age\u2010associated decline in immunity to infection has been documented across multiple pathogens, yet the relative contributions of the aged priming environment and of lymphocyte\u2010intrinsic defects remain unclear. To address the impact of the aging environment on T\u2010cell priming, adult na\u00efve OT\u2010I TCR transgenic CD8 T cells, specific for the H\u20102K<jats:sup>b<\/jats:sup>\u2010restricted immunodominant OVA<jats:sub>257\u2010264<\/jats:sub> epitope, were transferred into adult or old recipient mice infected with the recombinant intracellular bacterium <jats:italic>Listeria monocytogenes<\/jats:italic> carrying the chicken ovalbumin protein (Lm\u2010OVA). We consistently found that adult OT\u2010I CD8 expansion was reduced in aged recipient mice, and this correlated with numeric, phenotypic, and functional defects selectively affecting CD8\u03b1+ dendritic cells (DC). Following Lm\u2010OVA infection, aged mice failed to accumulate CD8\u03b1+ DC in the spleen, and these cells expressed much lower levels of critical costimulatory molecules in the first three days following infection. Further, aged CD8\u03b1+ DC showed impaired uptake of the bacteria at very early time points following infection. Treatment of aged mice with Flt3 ligand (Flt3L) improved the number of DC present in the spleen prior to Lm\u2010OVA infection, and improved, but did not reconstitute, OT\u2010I expansion to Lm\u2010OVA infection. These results suggest that age\u2010associated changes in antigen uptake, pathogen sensing, and\/or antigen presentation contribute to impaired adaptive immune responses to microbial pathogens with aging.<\/jats:p>","DOI":"10.1111\/j.1474-9726.2012.00867.x","type":"journal-article","created":{"date-parts":[[2012,8,3]],"date-time":"2012-08-03T18:04:41Z","timestamp":1344017081000},"page":"968-977","source":"Crossref","is-referenced-by-count":70,"title":["Age\u2010associated alterations in CD8\u03b1+ dendritic cells impair CD8 T\u2010cell expansion in response to an intracellular bacterium"],"prefix":"10.1111","volume":"11","author":[{"given":"Gang","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan J.","family":"Smithey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian D.","family":"Rudd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janko","family":"Nikolich\u2010\u017dugich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,8,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.11.6912"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.6.3725"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000568"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.4.1996"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20090222"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05581-11"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201041036"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.06.012"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2010.00149"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/bfgp\/4.3.258"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2012.04.001"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2004.00142.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2009.08.007"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00365-5"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/87720"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1064571"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2009.00879.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.5.1953"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2011.03.001"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.11.7977"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00883.x"},{"key":"e_1_2_7_23_1","first-page":"1165","article-title":"Phagocytosis and killing of bacteria by professional phagocytes and dendritic cells","volume":"9","author":"Nagl M","year":"2002","journal-title":"Clin. 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