{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T14:23:24Z","timestamp":1767795804121,"version":"3.49.0"},"reference-count":18,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2008,3,12]],"date-time":"2008-03-12T00:00:00Z","timestamp":1205280000000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ophthalmic Physiologic Optic"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Purpose:\u2002<\/jats:bold>\n                    To observe\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    cysts and trophozoites in the corneal tissue of patients and rabbits with\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    keratitis (AK), using the Heidelberg Retina Tomograph III Rostock\u2010cornea\u2010module (HRTIII\u2010RCM).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:\u2002<\/jats:bold>\n                    Eight eyes of seven patients with clinical and laboratory diagnosis of AK, presenting to the Beijing Institute of Ophthalmology between July and December 2006, were included in this study. The laboratory diagnosis in all cases was established by a cytological analysis and culture of the corneal scraping. The scanning with HRTIII\u2010RCM was performed on all the patients.\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    cysts and trophozoites in the corneas of three rabbits were observed with the HRTIII\u2010RCM after a suspension of trophozoites was injected into the corneal stromal layer of the rabbits.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:\u2002<\/jats:bold>\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    cysts or trophozoites were observed with the HRTIII\u2010RCM in the corneas of all the patients. The size and shape of\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    in the tissue of the rabbit corneas was consistent with that in the patients\u2019 eyes. The\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    cyst presented a round high\u2010contrast particle, 15\u201320\u2003\u03bcm in diameter, with a double wall. In contrast, the trophozoite presented an irregular high\u2010contrast particle, without an apparent wall, 25\u201340\u2003\u03bcm in diameter and with a round conspicuous nucleus.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:\u2002<\/jats:bold>\n                    HRTIII\u2010RCM is a useful non\u2010invasive clinical technique to help to diagnose and monitor the response of AK to therapy.\n                  <\/jats:p>","DOI":"10.1111\/j.1475-1313.2008.00540.x","type":"journal-article","created":{"date-parts":[[2008,3,13]],"date-time":"2008-03-13T00:36:51Z","timestamp":1205368611000},"page":"163-167","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Clinical and experimental findings in Acanthamoeba keratitis with Heidelberg Retina Tomograph III\u2010RCM"],"prefix":"10.1007","volume":"28","author":[{"given":"Zhang","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deng","family":"Shijing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wang","family":"Zhiqun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Ran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun","family":"Xuguang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2008,3,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-199403000-00012"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.eye.6702510"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/09273940590951098"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(93)31457-0"},{"key":"e_1_2_5_6_1","first-page":"197","article-title":"Early diagnosis of infectious keratitis with in vivo real time confocal microscopy","volume":"18","author":"Chew S. J.","year":"1992","journal-title":"CLAO J."},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6257(98)00004-6"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2003.12.002"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0181-5512(04)96252-3"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)70577-X"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1997.01100150716002"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.118.2.178"},{"key":"e_1_2_5_13_1","first-page":"1319","article-title":"The application of in vivo confocal scanning laser microscopy in the management of Acanthamoeba keratitis","volume":"25","author":"Matsumoto Y.","year":"2007","journal-title":"Mol. 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