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Samples were also collected from 45 clinically normal dogs to establish thromboelastometric reference intervals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Interventions<\/jats:title><jats:p>For each dog whole blood was collected by jugular venipuncture and placed into tubes containing 3.8% trisodium citrate and stored at 37\u00b0C. Two standard dilutions (1:4 and 1:10) from canine blood specimens were prepared with<jats:styled-content style=\"fixed-case\">HES<\/jats:styled-content>130\/0.4 and saline 0.9%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Measurements and Main Results<\/jats:title><jats:p>The effects of dilution were studied by<jats:styled-content style=\"fixed-case\">TEM<\/jats:styled-content>. No statistically significant differences were observed between the<jats:styled-content style=\"fixed-case\">TEM<\/jats:styled-content>values of the control samples and the samples diluted with saline solution (ratio 1:10 and 1:4). In contrast, hypocoagulability was observed in the samples treated with<jats:styled-content style=\"fixed-case\">HES<\/jats:styled-content>130\/0.4, with more severe effects at 1:4 dilution than at 1:10 dilution. The 1:4 dilution with HES 130\/0.4 produced marked alterations: CT (<jats:italic>P<\/jats:italic>= 0.035) and CFT (<jats:italic>P<\/jats:italic>= 0.0007) on the ex\u2010TEM profile, CT (<jats:italic>P<\/jats:italic>= 0.0005) and ML (<jats:italic>P<\/jats:italic>= 0.0017) on the fib\u2010TEM profile and CFT (<jats:italic>P<\/jats:italic>= 0.0043) on the in\u2010TEM, were all significantly increased (<jats:italic>P<\/jats:italic>&lt; 0.05), whereas MCF (<jats:italic>P<\/jats:italic>= 0.0042) and alpha angle (<jats:italic>P<\/jats:italic>= 0.002) on the in\u2010TEM and MCF (<jats:italic>P<\/jats:italic>= 0.011) and alpha angle (<jats:italic>P<\/jats:italic>= 0.001) ex\u2010TEM profiles and MCF (<jats:italic>P<\/jats:italic>= 0.0001) on the fib\u2010TEM profile were significantly decreased (<jats:italic>P<\/jats:italic>&lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Dilution of canine blood with<jats:styled-content style=\"fixed-case\">HES<\/jats:styled-content>130\/0.4 results in a thromboelastometric pattern consistent with hypocoagulation and this effect appears to result from a dose\u2010dependent alteration in fibrinogen concentration and inhibition of platelet function. 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