{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T22:06:17Z","timestamp":1791065177164,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,12,16]],"date-time":"2011-12-16T00:00:00Z","timestamp":1323993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:p><jats:bold>BACKGROUND AND PURPOSE<\/jats:bold> As prognosis for patients with metastatic ovarian cancer is generally poor, advances in treatment are needed. Here, we studied the mechanism of action of a recombinant viral capsid protein (rVP1) and explored its effect against ovarian tumour growth and metastasis <jats:italic>in vivo<\/jats:italic>.<\/jats:p><jats:p><jats:bold>EXPERIMENTAL APPROACH<\/jats:bold> The human ovarian cancer cell line SKOV3 and BALB\/cAnN\u2010Foxn1 female nude mice were used. Effects of rVP1 on the viability, invasive ability, matrix metalloproteinase (MMP)\u20102 activity and cancer cell proliferation and metastasis were determined by cell proliferation assay, Matrigel invasion assay, gelatin zymographic analysis, as well as bioluminescence imaging and immunohistological analysis in xenograft mouse models respectively. Levels of total and phosphorylated focal adhesion kinase (FAK), PKB\/Akt, phosphatase and tensin homologue (PTEN) and glycogen synthase kinase\u20103\u03b2 (GSK\u20103\u03b2) were detected by Western blotting.<\/jats:p><jats:p><jats:bold>KEY RESULTS<\/jats:bold> rVP1 promoted apoptosis and decreased invasion of human ovarian cancer cells. This effect of rVP1 was accompanied by activation of PTEN and GSK\u20103\u03b2 as well as down\u2010regulation of FAK, Akt and MMP\u20102. Anti\u2010integrin antibodies or overexpression of constitutively active Akt reversed the cellular effects of rVP1. Orthotopic and intraperitoneal xenograft mouse models demonstrated that rVP1 attenuated survival and metastasis of human ovarian cancer SKOV3 cell line <jats:italic>in vivo<\/jats:italic> through selective regulation of Akt and GSK\u20103\u03b2 activity as shown by bioluminescence imaging of mice and immunohistochemical analysis.<\/jats:p><jats:p><jats:bold>CONCLUSION AND IMPLICATIONS<\/jats:bold> These results indicate that negative regulation of Akt signalling and MMP\u20102 by rVP1 may have the potential to suppress ovarian tumour growth and metastasis <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1476-5381.2011.01581.x","type":"journal-article","created":{"date-parts":[[2011,7,14]],"date-time":"2011-07-14T10:07:04Z","timestamp":1310638024000},"page":"479-493","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Recombinant viral protein promotes apoptosis and suppresses invasion of ovarian 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