{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T01:56:53Z","timestamp":1793239013121,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,4,10]],"date-time":"2012-04-10T00:00:00Z","timestamp":1334016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:p><jats:bold>BACKGROUND AND PURPOSE<\/jats:bold>L\u2010tryptophan (L\u2010W) is a precursor of the vasoconstrictor, 5\u2010HT. However, acute administration of L\u2010W ethyl ester (L\u2010Wee) lowered blood pressure. The mechanism of action is unknown. This study compares the vascular effects of L\u2010W and L\u2010Wee in intact animals, isolated aortic rings, small mesenteric arteries (MA) and explores possible mechanisms by studies in vascular smooth muscle cells (VSMC) of MA.<\/jats:p><jats:p><jats:bold>EXPERIMENTAL APPROACH<\/jats:bold>Effects of L\u2010W or L\u2010Wee (5\u201350\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>, i.v.) on mean arterial pressure (MAP) and heart rate (HR) were determined in male Sprague\u2010Dawley rats. The effects of L\u2010W and L\u2010Wee on basal tone and of phenylephrine\u2010 or KCl\u2010induced contractions of aortic and MA rings were assessed. Effects of L\u2010Wee and L\u2010W on voltage\u2010operated calcium channels (VOCC) of VSMC of MA were also examined in patch\u2010clamp studies.<\/jats:p><jats:p><jats:bold>KEY RESULTS<\/jats:bold>Administration of L\u2010Wee, but not L\u2010W, evoked a rapid and transient dose\u2010dependent decrease in MAP and HR. While both agents failed to affect basal tone, L\u2010Wee decreased, concentration\u2010dependently, (I<jats:sub>max<\/jats:sub>&gt; 98%) tension responses to phenylephrine and KCl in an endothelium\u2010independent manner in aorta (IC<jats:sub>50<\/jats:sub>2\u2003mM) and MA (IC<jats:sub>50<\/jats:sub>17\u2003\u00b5M). L\u2010Wee evoked concentration\u2010dependent inhibition of VOCC currents (IC<jats:sub>50<\/jats:sub>12\u2003\u00b5M; I<jats:sub>max<\/jats:sub>90%) in VSMC of MA.<\/jats:p><jats:p><jats:bold>CONCLUSIONS AND IMPLICATIONS<\/jats:bold>Esterified L\u2010W (L\u2010Wee), but not L\u2010W, preferentially relaxed resistance vessels rather than conduit vessels. These effects were associated with blockade of VOCC by L\u2010Wee. Our findings suggest that the falls in MAP and HR induced by L\u2010Wee were due to blockade of VOCC by L\u2010Wee.<\/jats:p>","DOI":"10.1111\/j.1476-5381.2011.01693.x","type":"journal-article","created":{"date-parts":[[2011,9,28]],"date-time":"2011-09-28T01:20:34Z","timestamp":1317172834000},"page":"232-242","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["L\u2010Tryptophan ethyl ester dilates small mesenteric arteries by inhibition of voltage\u2010operated calcium channels in smooth 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