{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T00:46:03Z","timestamp":1793321163799,"version":"4.5.0"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,5,17]],"date-time":"2012-05-17T00:00:00Z","timestamp":1337212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:sec><jats:title>BACKGROUND AND PURPOSE<\/jats:title><jats:p>The 5\u2010ht<jats:sub>1E<\/jats:sub>receptor is highly expressed in the human brain and its structure is conserved in humans, suggesting an important physiological role for 5\u2010ht<jats:sub>1E<\/jats:sub>receptors. However, neither the function nor the distribution of this receptor has been characterized in the mammalian brain.<\/jats:p><\/jats:sec><jats:sec><jats:title>EXPERIMENTAL APPROACH<\/jats:title><jats:p>Rats and mice lack the 5\u2010ht<jats:sub>1E<\/jats:sub>receptor gene; thus, we used guinea pig brain tissue and immunofluorescent staining techniques to provide the first specific localization of 5\u2010ht<jats:sub>1E<\/jats:sub>receptors in the mammalian brain.<\/jats:p><\/jats:sec><jats:sec><jats:title>KEY RESULTS<\/jats:title><jats:p>High levels of 5\u2010ht<jats:sub>1E<\/jats:sub>receptors are detected in olfactory bulb glomeruli as well as the molecular layer of the dentate gyrus (DG). In DG membranes, BRL54443, a 5\u2010ht<jats:sub>1E<\/jats:sub>\/5\u2010HT<jats:sub>1F<\/jats:sub>agonist, selectively stimulated 5\u2010ht<jats:sub>1E<\/jats:sub>receptors and potently inhibited forskolin\u2010dependent cAMP production (IC<jats:sub>50<\/jats:sub>= 14\u2003nM). The staining pattern of 5\u2010ht<jats:sub>1E<\/jats:sub>receptors in brain tissue suggests that this receptor is expressed predominantly in neurons rather than in glia. Additionally, 5\u2010ht<jats:sub>1E<\/jats:sub>receptors were detected in the adventitial layer of cerebral arteries but not in the microvasculature, venous tissue or other brain arteries.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS AND IMPLICATIONS<\/jats:title><jats:p>These observations should help to predict clinical effects of 5\u2010ht<jats:sub>1E<\/jats:sub>\u2010selective drugs. For example, the stimulation of 5\u2010ht<jats:sub>1E<\/jats:sub>receptors and subsequent inhibition of adenylate cyclase activity in the DG suggests that 5\u2010ht<jats:sub>1E<\/jats:sub>receptors may mediate regulation of hippocampal activity by 5\u2010HT, making it a possible drug target for the treatment of neuropsychiatric disorders characterized by memory deficits (such as Alzheimer's disease) or as a target for the treatment of temporal lobe epilepsy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1476-5381.2012.01868.x","type":"journal-article","created":{"date-parts":[[2012,1,20]],"date-time":"2012-01-20T06:13:41Z","timestamp":1327040021000},"page":"1290-1302","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Distribution of 5\u2010ht<sub>1E<\/sub>receptors in the mammalian brain and cerebral vasculature: an immunohistochemical and pharmacological 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