{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T12:03:36Z","timestamp":1780401816771,"version":"3.54.1"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,12,18]],"date-time":"2012-12-18T00:00:00Z","timestamp":1355788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p><jats:bold>BACKGROUND AND PURPOSE<\/jats:bold> Sirtuin 6 (SIRT6) is involved in regulation of glucose and fat metabolism. However, its possible contribution to cardiac dysfunction remains to be determined. In the present study, the effect of SIRT6 on cardiac hypertrophy induced by angiotensin II (AngII) and the underlying molecular mechanisms were investigated.<\/jats:p><jats:p><jats:bold>EXPERIMENTAL APPROACH<\/jats:bold> The expression and deacetylase activity of SIRT6 were measured in hypertrophic cardiomyocytes induced by AngII. After SIRT6 overexpression by transfection, or depletion by RNA interference in neonatal rat cardiomyocytes, cellular hypertrophy was monitored by measuring cell surface area and the mRNA levels of hypertrophic biomarkers. Further, the interaction between SIRT6 and the transcription factor NF\u2010\u03baB was investigated by co\u2010immunoprecipitation, confocal immunofluorescence microscopy and luciferase reporter gene assay. The expression and deacetylase activity of SIRT6 were measured <jats:italic>in vivo<\/jats:italic>, using the abdominal aortic constriction (AAC) model of cardiac hypertrophy in rats.<\/jats:p><jats:p><jats:bold>KEY RESULTS<\/jats:bold> In AngII\u2010induced hypertrophic cardiomyocytes and also in AAC\u2010induced hypertrophic hearts, the expression of SIRT6 protein was upregulated, while its deacetylase activity was decreased. Overexpression of wild\u2010type SIRT6 but not its catalytically inactive mutant, attenuated AngII\u2010induced cardiomyocyte hypertrophy. We further demonstrated a physical interaction between SIRT6 and NF\u2010\u03baB catalytic subunit p65, whose transcriptional activity could be repressed by SIRT6 overexpression.<\/jats:p><jats:p><jats:bold>CONCLUSIONS AND IMPLICATIONS<\/jats:bold> Our findings suggest that SIRT6 suppressed cardiomyocyte hypertrophy <jats:italic>in vitro<\/jats:italic> via inhibition of NF\u2010\u03baB\u2010dependent transcriptional activity and that this effect was dependent on its deacetylase activity.<\/jats:p>","DOI":"10.1111\/j.1476-5381.2012.01903.x","type":"journal-article","created":{"date-parts":[[2012,2,15]],"date-time":"2012-02-15T16:55:24Z","timestamp":1329324924000},"page":"117-128","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":90,"title":["Sirtuin 6 protects cardiomyocytes from hypertrophy <i>in vitro<\/i> via inhibition of NF\u2010\u03baB\u2010dependent transcriptional 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