{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:00:05Z","timestamp":1787014805453,"version":"build-2736575974"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,8,14]],"date-time":"2012-08-14T00:00:00Z","timestamp":1344902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet Comparative Oncology"],"published-print":{"date-parts":[[2014,6]]},"abstract":"Abstract<\/jats:title>\n \n Here, we describe the establishment of mutant\u2010specific polymerase chain reaction (\n PCR<\/jats:styled-content>\n ) for detection of a\n \n c\u2010\n KIT<\/jats:styled-content>\n <\/jats:italic>\n c.\n 1430G<\/jats:styled-content>\n >T mutation in feline mast cell tumours. Several mutations in feline\n \n c\u2010\n KIT<\/jats:styled-content>\n <\/jats:italic>\n have been identified, with the c.\n 1430G<\/jats:styled-content>\n >T mutation accounting for a significant portion of feline mast cell tumour mutations. The c.\n 1430G<\/jats:styled-content>\n >T mutation in\n \n c\u2010\n KIT<\/jats:styled-content>\n <\/jats:italic>\n exon 9 was detected in 15.7% (11 of 70) of samples by mutant\u2010specific\n PCR<\/jats:styled-content>\n but in only 7.1% (5 of 70) by\n PCR<\/jats:styled-content>\n \u2013restriction fragment length polymorphism (\n RFLP<\/jats:styled-content>\n ) in the genomic\n DNA<\/jats:styled-content>\n isolated from 70 formalin\u2010fixed paraffin\u2010embedded sections or cells collected by fine needle aspiration. Mutant\u2010specific\n PCR<\/jats:styled-content>\n showed remarkably higher detection rate than did\n PCR\u2013RFLP<\/jats:styled-content>\n .\n DNA<\/jats:styled-content>\n sequence analysis did not always yield identical results to those of mutant\u2010specific\n PCR<\/jats:styled-content>\n , suggesting heterogeneity of tumour cells. Mutant\u2010specific\n PCR<\/jats:styled-content>\n is a valid and efficient screening tool for detection of the\n \n c\u2010\n KIT<\/jats:styled-content>\n <\/jats:italic>\n c.\n 1430G<\/jats:styled-content>\n >T point mutation in feline mast cell tumours compared with\n PCR\u2013RFLP<\/jats:styled-content>\n and sequencing analysis.\n <\/jats:p>","DOI":"10.1111\/j.1476-5829.2012.00346.x","type":"journal-article","created":{"date-parts":[[2012,8,14]],"date-time":"2012-08-14T10:47:39Z","timestamp":1344941259000},"page":"138-142","source":"Crossref","is-referenced-by-count":6,"title":["Sensitive detection of the\n \n c\u2010\n KIT<\/scp>\n <\/i>\n c<\/i>\n .\n 1430G<\/scp>\n >T mutation by mutant\u2010specific polymerase chain reaction in feline mast cell tumours"],"prefix":"10.1111","volume":"12","author":[{"given":"M.","family":"Takanosu","sequence":"first","affiliation":[{"name":"Nasunogahara Animal Clinic Ohtawara Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Sato","sequence":"additional","affiliation":[{"name":"D\u2010Lab Sapporo, Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Kagawa","sequence":"additional","affiliation":[{"name":"North Lab Sapporo, Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,8,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2006.060089"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/hupa.2002.124124"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2006.07.004"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2008.00132.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2009.07926.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-08-0067"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.2741\/1785"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5810.2004.00059.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.tcam.2009.02.002"},{"key":"e_1_2_5_11_1","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1038\/sj.onc.1202171","article-title":"Tyrpsine kinase receptor\u2010activated singal transduction pathways which lead to oncogenesis","volume":"17","author":"Porter AC","year":"1998","journal-title":"Oncogenes"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2004.05.006"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00019606-199702000-00008"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2007.047928"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4914(01)02217-1"},{"key":"e_1_2_5_16_1","first-page":"61","article-title":"Intra\u2010tumoral heterogeneity of KRAS and BRAF mutation status in patients with advanced colorectal cancer (aCRC) and cost\u2010effectiveness of multiple sample testing 1","volume":"34","author":"Richman SD","year":"2011","journal-title":"Cancer Biology & Therapy"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029336"}],"container-title":["Veterinary and Comparative Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1476-5829.2012.00346.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1476-5829.2012.00346.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T06:25:39Z","timestamp":1696141539000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1476-5829.2012.00346.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,14]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1111\/j.1476-5829.2012.00346.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1476-5829.2012.00346.x","archive":["Portico"],"relation":{},"ISSN":["1476-5810","1476-5829"],"issn-type":[{"value":"1476-5810","type":"print"},{"value":"1476-5829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,14]]}}}