{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T05:41:54Z","timestamp":1697175714053},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,11,26]],"date-time":"2004-11-26T00:00:00Z","timestamp":1101427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:p><jats:bold>Abstract: Background: <\/jats:bold> Chemokines and their receptors are important mediators of leucocyte trafficking and are suggested to be critical for establishment of inflammatory autoimmune processes. CC chemokine receptor 5 (CCR5) is expressed preferentially by CD4+ T cells. We hypothesised that the CCR5delta(\u0394)32 genotype, which impairs surface expression of CCR5 in heterozygotes and is linked to a functional polymorphism of CD45RA expressed on suppressor\u2010inducer\u2010like \u2018na\u00efve\u2019 CD4+ T cells, may modulate the inflammatory process in primary biliary cirrhosis (PBC).<\/jats:p><jats:p><jats:bold>Methods: <\/jats:bold> CCR5\u039432 polymorphism was determined by PCR in 226 Caucasian PBC patients and 197 racially matched controls from two geographical areas, Newcastle, UK and Padua, Italy. (UK: 144 PBC, 105 controls, Italy: 82 PBC, 92 controls).<\/jats:p><jats:p><jats:bold>Results: <\/jats:bold> When the two series were analysed separately, there were no significant differences in the genotype distribution comparing patients and controls (UK: wt\/wt 72% vs 76%; wt\/\u039432 28% vs 22%; \u039432\/\u039432 0% vs 2%, <jats:italic>P<\/jats:italic>=0.24; Italy: wt\/wt 72% vs 82%; wt\/\u039432 27% vs 17%; \u039432\/\u039432 0% vs 1%, <jats:italic>P<\/jats:italic>=0.14). However, when the data for the two series were pooled and reanalysed, we found an increase in the CCR5\u039432 mutation in PBC patients vs controls (28% vs 21%, OR=1.43, <jats:italic>P<\/jats:italic>=0.03), but there was no evidence that this \u039432 polymorphism is associated with less severe disease.<\/jats:p><jats:p><jats:bold>Conclusions: <\/jats:bold> Although this two\u2010centre genetic association study is large compared with others performed in PBC, taken separately, each geographically based cohort of patients and controls is underpowered to detect a small effect of this functional polymorphism. This emphasises the need for far larger case\u2013control collections to address which polymorphic markers or haplotypes might modify the pathogenesis and clinical course of PBC. We propose that multi\u2010centre collaboration on an international scale in \u2018orphan\u2019 complex liver diseases such as (PBC) is supported by the International Association for the Study of the Liver and promoted via their journal with development of a brief format for web\u2010based publication of studies.<\/jats:p>","DOI":"10.1111\/j.1478-3231.2004.0980.x","type":"journal-article","created":{"date-parts":[[2004,11,26]],"date-time":"2004-11-26T13:36:39Z","timestamp":1101476199000},"page":"646-650","source":"Crossref","is-referenced-by-count":4,"title":["Chemokine receptor 5 and primary biliary cirrhosis: a two\u2010centre genetic association study"],"prefix":"10.1111","volume":"24","author":[{"given":"A.","family":"Baragiotta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Floreani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Agarwal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Venturi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Craggs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. E. J.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. T.","family":"Donaldson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. F.","family":"Bassendine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,11,26]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-9034-1_17"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1089-3261(03)00095-3"},{"key":"e_1_2_7_4_2","first-page":"402","article-title":"Familial primary biliary cirrhosis reassessed","volume":"30","author":"Jones D E","year":"1999","journal-title":"a geographically-based population study"},{"key":"e_1_2_7_5_2","first-page":"387A","article-title":"Occurrence of primary biliary cirrhosis in monozygotic twins","volume":"36","author":"Selmi C","year":"2002","journal-title":"Hepatology"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199410063311418"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2001.29760"},{"key":"e_1_2_7_8_2","first-page":"586","article-title":"PBC","volume":"47","author":"Haydon G H","year":"2000","journal-title":"an infectious disease?"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20024"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1993.tb03397.x"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/33340"},{"key":"e_1_2_7_12_2","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1046\/j.1440-1711.2002.01062.x","article-title":"Recruitment of lymphocytes to the human liver","volume":"80","author":"Lalor P F","year":"2003","journal-title":"Immunol Cell Biol"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324285"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000112595.65972.8A"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108321200"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.1.148"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1995.tb05538.x"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365520310000843"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2003.03165.x"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1159\/000048605"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-135-9-200111060-00008"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1468-1293.2002.00106.x"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002510050621"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/lt.20095"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01621"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81110-1"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/8702"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.2001.0538"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20159"},{"key":"e_1_2_7_30_2","first-page":"355","article-title":"TNF polymorphisms in PBC","volume":"31","author":"Donaldson P T.","year":"1999","journal-title":"a critical approach"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)12715-8"}],"container-title":["Liver International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1478-3231.2004.0980.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1478-3231.2004.0980.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T07:16:58Z","timestamp":1697095018000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1478-3231.2004.0980.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,11,26]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["10.1111\/j.1478-3231.2004.0980.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1478-3231.2004.0980.x","archive":["Portico"],"relation":{},"ISSN":["1478-3223","1478-3231"],"issn-type":[{"value":"1478-3223","type":"print"},{"value":"1478-3231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,11,26]]}}}