{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T17:18:02Z","timestamp":1789924682020,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,5,20]],"date-time":"2005-05-20T00:00:00Z","timestamp":1116547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background: <\/jats:bold> Primary biliary cirrhosis (PBC) is one of the organ\u2010specific autoimmune diseases characterized by destruction of the biliary epithelial cells, cholestasis, liver cirrhosis, and liver failure. With the postulation that induction of the autoimmune process might induce PBC\u2010like cholangitis, here we used polyinosinic polycytidylic acid (poly I:C), an inducer of type\u20101 interferon (IFN), to generate an autoimmune cholangitis animal model.<\/jats:p><jats:p><jats:bold>Methods: <\/jats:bold> Female C57BL\/6 mice were injected with 5\u2003mg\/kg of poly I:C twice a week for 28 consecutive weeks. Liver specimens were collected to evaluate the degree of cell infiltration. Autoantibodies, including antimitochondrial antibody (AMA), were assayed by immunofluorescence, enzyme\u2010linked immunosorbent assay (ELISA) and immunoblotting. IFN\u2010\u03b1 was estimated in the sera by an ELISA method. Poly I:C injection induced IFN\u2010\u03b1.<\/jats:p><jats:p><jats:bold>Results: <\/jats:bold> Mononuclear cells were detected at the portal areas 8 weeks after the start of poly I:C injection, which progressed up to 16 weeks. Autoantibodies, including AMA, were detected in the sera from all poly I:C\u2010injected mice.<\/jats:p><jats:p><jats:bold>Conclusions: <\/jats:bold> In conclusion, we show an early development of a PBC\u2010like cholangitis in a genetically susceptible mouse strain because of poly I:C administration. This model would be helpful to study PBC immunopathogenesis and to evaluate the effectiveness of newly developed therapeutic regimens for PBC.<\/jats:p>","DOI":"10.1111\/j.1478-3231.2005.01043.x","type":"journal-article","created":{"date-parts":[[2005,5,20]],"date-time":"2005-05-20T21:58:29Z","timestamp":1116626309000},"page":"595-603","source":"Crossref","is-referenced-by-count":47,"title":["Early development of primary biliary cirrhosis in female C57BL\/6 mice because of poly I:C administration"],"prefix":"10.1111","volume":"25","author":[{"given":"Chizuko","family":"Okada","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sk. Md. 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