{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:44:52Z","timestamp":1790685892062,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,18]],"date-time":"2005-11-18T00:00:00Z","timestamp":1132272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:p><jats:bold>Abstract: Background\/Aims: <\/jats:bold> Hepatocytes (HEP) have been the major target for structural quantification in the liver, but an estimation of their total number (<jats:italic>N<\/jats:italic>), their percentage in relation to the global number of liver cells and the evaluation of the percentage of binucleated hepatocytes (BnHEPs) have never been performed with modern design\u2010based stereological techniques. The establishment of sound technical guidelines and baseline quantitative data in non\u2010pathological conditions are relevant to properly evaluate HEP hyperplasia and BnHEP responses.<\/jats:p><jats:p><jats:bold>Methods: <\/jats:bold> In this study, we combined immunocytochemistry with sound design\u2010based stereology for estimating the <jats:italic>N<\/jats:italic> of HEP and the <jats:italic>N<\/jats:italic> of non\u2010hepatocytic cells (NHCs). For obtaining systematic uniform random sections (30\u2003\u03bcm thick), a smooth fractionator sampling scheme was applied to the liver of five male Wistar rats (3 month old). Those sections were immunostained with polyclonal antibodies against carcinoembryonic antigen. Because biliary canaliculi were then marked, an unequivocal counting of mononucleated hepatocytes (MnHEP) and BnHEP was allowed.<\/jats:p><jats:p><jats:bold>Results: <\/jats:bold> The <jats:italic>N<\/jats:italic> of HEP was estimated to be 1.93 \u00d7 10<jats:sup>9<\/jats:sup>, with a coefficient of error (CE) of 0.02, corresponding to 129 \u00d7 10<jats:sup>6<\/jats:sup>\u2003HEP\/g of liver. BnHEP represented 26% of total HEP number. The <jats:italic>N<\/jats:italic> of NHC was estimated as 1.31 \u00d7 10<jats:sup>9<\/jats:sup> (CE=0.02).<\/jats:p><jats:p><jats:bold>Conclusion: <\/jats:bold> The strategy here presented provides a reliable method for accessing the <jats:italic>N<\/jats:italic> of HEP (distinguishing MnHEP from BnHEP) in situations in which these parameters are relevant, namely for evaluating the magnitude of an hyperplastic liver response from its very early onset.<\/jats:p>","DOI":"10.1111\/j.1478-3231.2005.01201.x","type":"journal-article","created":{"date-parts":[[2005,11,18]],"date-time":"2005-11-18T14:48:02Z","timestamp":1132325282000},"page":"116-124","source":"Crossref","is-referenced-by-count":39,"title":["Design\u2010based stereological estimation of hepatocyte number, by combining the smooth optical fractionator and immunocytochemistry with anti\u2010carcinoembryonic antigen polyclonal antibodies"],"prefix":"10.1111","volume":"26","author":[{"given":"Ricardo","family":"Marcos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rog\u00e9rio A. 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