{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T15:26:31Z","timestamp":1770218791000,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,5,11]],"date-time":"2006-05-11T00:00:00Z","timestamp":1147305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:p><jats:bold>Abstract: Background\/aims:<\/jats:bold>The purpose of this study was to validate spontaneous chronic hepatitis and cirrhosis in dogs as a potential large animal model for fibrotic liver disease in humans by evaluating their molecular pathophysiology.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold>Transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21) signalling was analysed in liver samples of dogs with acute hepatitis (AH), chronic hepatitis (CH), cirrhosis (CIRR), and a specific form of cirrhosis, lobular dissecting hepatitis (LDH), in comparison with human cirrhotic samples from alcohol abuse (ALC) and hepatitis C (HC).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold>Canine samples were investigated with quantitative real\u2010time PCR (Q\u2010PCR) and Western blotting on TGF\u2010\u03b21 signalling including Smad2\/3 phosphorylation. Immunohistochemistry on collagens I and III was performed. Q\u2010PCR showed an increase in TGF\u2010\u03b21 levels and downstream effector gene products in CH, LDH, and CIRR. The same fibrotic diseases also showed an increase in phosphorylated Smad2\/3 and a higher deposition of collagens I and III. In contrast, in AH neither active TGF\u2010\u03b21 signalling nor collagen deposition was observed. Western blot analysis on human ALC and HC indicated a high similarity with canine samples in TGF\u2010\u03b21 expression and Smad2\/3 phosphorylation.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold>Our results demonstrate that fibrosis in spontaneous dog liver diseases is highly comparable to their human counterparts and might serve as models for anti\u2010fibrotic strategies.<\/jats:p>","DOI":"10.1111\/j.1478-3231.2006.01277.x","type":"journal-article","created":{"date-parts":[[2006,5,11]],"date-time":"2006-05-11T15:54:39Z","timestamp":1147362879000},"page":"716-725","source":"Crossref","is-referenced-by-count":25,"title":["Transforming growth factor \u03b2\u20101 signalling in canine hepatic diseases: new models for human fibrotic liver pathologies"],"prefix":"10.1111","volume":"26","author":[{"given":"Bart","family":"Spee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitte","family":"Arends","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ted S. 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