{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T13:05:13Z","timestamp":1783602313851,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,3,14]],"date-time":"2007-03-14T00:00:00Z","timestamp":1173830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\/Aims: <\/jats:bold> Reactive bile ductule is a non\u2010specific feature of various hepatobiliary diseases, and is not infrequently accompanied by neutrophilic infiltration. Recently, biliary epithelial cells have been shown to secrete cytokines and chemokines and to express components of the mucosal immune system such as Toll\u2010like receptors.<\/jats:p><jats:p><jats:bold>Methods and Results: <\/jats:bold> We examined the expression of a neutrophil chemo\u2010attractant, interluekin (IL)\u20108, in bile ductular cells to clarify the histogenesis of reactive bile ductules with neutrophilic infiltration using human liver tissues (eight cases of chronic viral hepatitis, seven cases of liver cirrhosis (LC), seven cases of sepsis, 11 cases of extrahepatic biliary obstruction (EBO), three cases of fulminant hepatitis (FH), five cases of primary biliary cirrhosis, and three cases of primary sclerosing cholangitis). Human neutrophil peptides 1\u20133 (HNP1\u20133) were used as markers of neutrophils. Immunohistochemically, IL\u20108 was detected in bile ductules in various diseased livers. HNP1\u20133\u2010positive neutrophils were significantly dense around IL\u20108\u2010positive bile ductules compared with IL\u20108\u2010negative ductules in septic liver, LC, EBO, and FH. Experiments <jats:italic>in vitro<\/jats:italic> showed that cultured human biliary epithelial cells expressed and secreted IL\u20108 in response to lipopolysaccharide and also IL\u20101\u03b2 and tumour necrosis factor\u2010\u03b1.<\/jats:p><jats:p><jats:bold>Conclusions: <\/jats:bold> Neutrophilic infiltration around reactive bile ductules may be related to the IL\u20108 expressed in bile ductular epithelia, possibly induced by bacterial components and proinflammatory cytokines released locally.<\/jats:p>","DOI":"10.1111\/j.1478-3231.2007.01465.x","type":"journal-article","created":{"date-parts":[[2007,3,15]],"date-time":"2007-03-15T01:58:05Z","timestamp":1173923885000},"page":"672-680","source":"Crossref","is-referenced-by-count":55,"title":["IL\u20108 expression by biliary epithelial cells is associated with neutrophilic infiltration and reactive bile ductules"],"prefix":"10.1111","volume":"27","author":[{"given":"Kumiko","family":"Isse","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenichi","family":"Harada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuni","family":"Nakanuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,3,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(19970915)38:6<552::AID-JEMT2>3.0.CO;2-H"},{"key":"e_1_2_6_3_2","first-page":"1019","article-title":"Neuroendocrine features of reactive bile ductules in cholestatic liver disease","volume":"137","author":"Roskams T","year":"1990","journal-title":"Am J Pathol"},{"key":"e_1_2_6_4_2","first-page":"1739","article-title":"Nomenclature of the finer branches of the biliary 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