{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:38:50Z","timestamp":1789205930383,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2011,8,7]],"date-time":"2011-08-07T00:00:00Z","timestamp":1312675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>Alternative and\/or complementary sources of cells such as hepatic progenitor cells (<jats:styled-content style=\"fixed-case\">HPC<\/jats:styled-content>) are under investigation for hepatic cell therapy purposes. Steatotic livers are those most commonly rejected for clinical transplantation and are also unsuitable for good quality hepatocyte isolation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim:<\/jats:title><jats:p>Taken together these two facts, our aim was to investigate whether they could represent a suitable source for the isolation of progenitor cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Rats fed for 7\u00a0weeks with methionine\u2013choline deficient diets showing proved steatotic signs (i.e. increase in hepatic lipids; macrovesicular steatosis) and steatotic and normal human liver samples were used to study the expression of <jats:styled-content style=\"fixed-case\">HPC<\/jats:styled-content> markers and to isolate these cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>In the liver of the steatotic rats there was a significant increase in <jats:styled-content style=\"fixed-case\">HPC<\/jats:styled-content> (known as oval cells in rodents) markers such as <jats:styled-content style=\"fixed-case\">Thy<\/jats:styled-content>\u20101, epithelial cell adhesion molecule (<jats:styled-content style=\"fixed-case\">EpCAM<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">OV<\/jats:styled-content>\u20106 (2\u2010, 3\u2010 and 5\u2010fold increase respectively). Additionally, there was an increase in the yield of isolated oval cells compared to control rats. Similarly, studies using human livers clearly confirmed an increase in the expression of <jats:styled-content style=\"fixed-case\">HPC<\/jats:styled-content> markers in the steatotic tissue and a significant rise in the number of isolated progenitor cells (<jats:styled-content style=\"fixed-case\">EpCAM<\/jats:styled-content>+, <jats:styled-content style=\"fixed-case\">Thy<\/jats:styled-content>\u20101+, <jats:styled-content style=\"fixed-case\">OV<\/jats:styled-content>\u20106+) (10, 12 and 11.6\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup>\u00a0cells\/g of tissue respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>These data suggest that steatotic livers, discarded for orthotopic liver transplantation and hepatocyte isolation, could be a suitable source for large scale isolation of <jats:styled-content style=\"fixed-case\">HPC<\/jats:styled-content> which might be potential candidates in liver cell therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1478-3231.2011.02609.x","type":"journal-article","created":{"date-parts":[[2011,8,8]],"date-time":"2011-08-08T04:09:38Z","timestamp":1312776578000},"page":"1231-1238","source":"Crossref","is-referenced-by-count":14,"title":["Steatotic liver: a suitable source for the isolation of hepatic progenitor cells"],"prefix":"10.1111","volume":"31","author":[{"given":"Laia","family":"Tolosa","sequence":"first","affiliation":[{"name":"Unidad de Hepatolog\u00eda Experimental Centro de Investigaci\u00f3n Hospital La Fe Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Bonora\u2010Centelles","sequence":"additional","affiliation":[{"name":"Unidad de Hepatolog\u00eda Experimental Centro de Investigaci\u00f3n Hospital La Fe Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Teresa Donato","sequence":"additional","affiliation":[{"name":"Unidad de Hepatolog\u00eda 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