{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T11:38:01Z","timestamp":1774352281042,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,4,12]],"date-time":"2012-04-12T00:00:00Z","timestamp":1334188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Liver International"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title><jats:italic>Background<\/jats:italic><\/jats:title><jats:p>Although the migration of hepatic stellate cells (<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s) is essential to the hepatic fibrotic response, the intracellular and extracellular signals that regulate their migration are poorly understood.<\/jats:p><\/jats:sec><jats:sec><jats:title><jats:italic>Aims<\/jats:italic><\/jats:title><jats:p>To investigate the role of Rho guanosine triphosphatase (Rho <jats:styled-content style=\"fixed-case\">GTP<\/jats:styled-content>ase) signalling, specifically via RhoA, in transforming growth factor \u03b21 (<jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u03b21)\u2010induced <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content> migration.<\/jats:p><\/jats:sec><jats:sec><jats:title><jats:italic>Methods<\/jats:italic><\/jats:title><jats:p>Both primary rat <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s and the <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>\u2010T6 rat hepatic stellate cell line were used in this study. Cell migration was evaluated using the Transwell Boyden Chamber assay, whereas cytoskeletal changes were observed using laser confocal microscopy. Western blotting was used to detect the expression of Rho <jats:styled-content style=\"fixed-case\">GTP<\/jats:styled-content>ases (RhoA, Rac1 and Cdc42) in <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s, and their activation was determined using glutathione S\u2010transferase (<jats:styled-content style=\"fixed-case\">GST<\/jats:styled-content>) pull\u2010down assays. Finally, the specific effects of RhoA on <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u03b21\u2010induced cell migration were analysed in <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>\u2010T6 cells stably transfected with constitutively active (<jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>, Q63L) or dominant\u2010negative (<jats:styled-content style=\"fixed-case\">DN<\/jats:styled-content>, T19N) RhoA mutants.<\/jats:p><\/jats:sec><jats:sec><jats:title><jats:italic>Results<\/jats:italic><\/jats:title><jats:p>Transforming growth factor \u03b21 induced cytoskeletal remodelling and migration of rat <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s following RhoA activation. The level of RhoA activation determined the motility of the <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title><jats:italic>Conclusions<\/jats:italic><\/jats:title><jats:p>These findings broaden our understanding of the intracellular and extracellular signals that regulate <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content> migration. Furthermore, RhoA may be a candidate therapeutic target for hepatic fibrosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1478-3231.2012.02809.x","type":"journal-article","created":{"date-parts":[[2012,4,13]],"date-time":"2012-04-13T02:13:52Z","timestamp":1334283232000},"page":"1093-1102","source":"Crossref","is-referenced-by-count":25,"title":["Effect of Rho<scp>A<\/scp> on transforming growth factor \u03b21\u2010induced rat hepatic stellate cell migration"],"prefix":"10.1111","volume":"32","author":[{"given":"Lei","family":"Li","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Zhongshan Hospital, Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Yao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Zhongshan Hospital, Fudan University  Shanghai 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