{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T21:21:16Z","timestamp":1783545676763,"version":"3.55.0"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,8,5]],"date-time":"2005-08-05T00:00:00Z","timestamp":1123200000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:bold>Background.\u2002<\/jats:bold> The innocuous pure recombinant cholera toxin B\u2010subunit (rCTB) is very attractive as a strong adjuvant for host immunization, but little is known about rCTB's gastric mucosal immunoadjuvanticity against <jats:italic>Helicobacter pylori<\/jats:italic>. The immunoadjuvanticity of rCTB against <jats:italic>H.\u00a0pylori<\/jats:italic> was tested.<\/jats:p><jats:p><jats:bold>Material and methods.\u2002<\/jats:bold> Mice were immunized with sonicated <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> and rCTB orally or intranasally and sacrificed on day 42 after immunization. Passive cutaneous anaphylaxis (PCA) test was performed to evaluate IgE\u2010mediated anaphylaxis with serum from mice to which <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010antigen with rCTB had been administered. Immunoglobulin titer specific to <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> in serum, lavation of the gastrointestinal tracts and feces were examined. Gastritis in vaccinated mice after a challenge was assessed with the scoring defined from grading of gastric inflammation. <jats:italic>H.\u00a0pylori<\/jats:italic> proliferation after immunization was investigated by counting colony forming units (CFU) per gram of stomach tissue.<\/jats:p><jats:p><jats:bold>Results.\u2002<\/jats:bold> PCA test exhibited no reactions against the serum from mice immunized with <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic>\u2010antigen with rCTB administered orally and intranasally. Oral and nasal coadministrations of rCTB significantly raised systemic and mucosal immunities against <jats:italic>H.\u00a0pylori<\/jats:italic> and suppressed proliferation of <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> in gastric mucosa. The score of gastritis in mice immunized orally was significantly higher than that of mice immunized nasally due to postimmunization gastritis. Only oral administration of rCTB suppressed <jats:italic>H.\u00a0pylori<\/jats:italic> proliferation as compared with intranasal administration and without rCTB.<\/jats:p><jats:p><jats:bold>Conclusions.\u2002<\/jats:bold> The present study indicated that rCTB has systemic and mucosal immunoadjuvanticities against <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> and that oral vaccination with rCTB might additively support antibiotic eradication.<\/jats:p>","DOI":"10.1111\/j.1523-5378.2005.00328.x","type":"journal-article","created":{"date-parts":[[2005,8,5]],"date-time":"2005-08-05T09:22:13Z","timestamp":1123233733000},"page":"345-352","source":"Crossref","is-referenced-by-count":9,"title":["Oral Vaccination Against <i>Helicobacter pylori<\/i> with Recombinant Cholera Toxin 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