{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:55:58Z","timestamp":1761058558578},"reference-count":82,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,11,2]],"date-time":"2006-11-02T00:00:00Z","timestamp":1162425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background:\u2002<\/jats:bold> Several <jats:italic>Helicobacter pylori<\/jats:italic> proteins have been reported to be associated with severe symptoms of gastric disease. However, expression levels of most of these disease\u2010associated proteins require further evaluation in order to clarify their relationships with gastric disease patterns. Representative proteome components of 71 clinical isolates of <jats:italic>H.\u00a0pylori<\/jats:italic> were analyzed quantitatively to determine whether the protein expression levels were associated with gastric diseases and to cluster clinical isolates.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> After two\u2010dimensional electrophoresis (2\u2010DE) of <jats:italic>H.\u00a0pylori<\/jats:italic> isolates, spot intensities were analyzed using <jats:sc>pdquest<\/jats:sc> 2\u2010D Gel Analysis Software. The intensities of 10 representative protein spots, identified by peptide fingerprinting using matrix assisted laser desorption ionization\u2010time of flight mass spectrometry (MALDI\u2010TOF\u2010MS) or peptide sequencing using quadrupole TOF MS, were subjected to the nonparametric Mann\u2013Whitney test and hierarchical agglomerative cluster analysis. The relationship between clusters and gastric diseases was analyzed by the chi\u2010squared test.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Although the spot intensities of the 10 representative proteins were highly variable within each gastric disease group, the expression levels of CagA, UreB, GroEL, EF\u2010Tu, EF\u2010P, TagD, and FldA showed some significant differences among the gastric disease patterns. On the basis of the 10 target protein intensities, hierarchical agglomerative cluster analysis generated a dendrogram with clusters indicative of chronic gastritis\/gastric cancers and gastric\/duodenal ulcers.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> These results indicated that quantitative analysis of proteome components is a feasible method for examining disease\u2010associated proteins and clustering clinical strains of <jats:italic>H.\u00a0pylori<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1523-5378.2006.00456.x","type":"journal-article","created":{"date-parts":[[2006,11,2]],"date-time":"2006-11-02T20:25:07Z","timestamp":1162499107000},"page":"533-543","source":"Crossref","is-referenced-by-count":22,"title":["Quantitative Analysis of Representative Proteome Components and Clustering of <i>Helicobacter pylori<\/i> Clinical Strains"],"prefix":"10.1111","volume":"11","author":[{"given":"Jeong\u2010Won","family":"Park","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae\u2010Young","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung\u2010Gyu","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin\u2010Su","family":"Jun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong\u2010Uck","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi\u2010Ja","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung\u2010Soo","family":"Ju","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damir","family":"Nizamutdinov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myung\u2010Woong","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hee\u2010Shang","family":"Youn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyung\u2010Lyun","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung\u2010Chul","family":"Baik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woo\u2010Kon","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myung\u2010Je","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwang\u2010Ho","family":"Rhee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,11,2]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1016\/S0140-6736(84)91816-6","article-title":"Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration","volume":"16","author":"Marshall BJ","year":"1984","journal-title":"Lancet"},{"key":"e_1_2_6_3_2","first-page":"455","article-title":"Prevalence of Helicobacter pylori infection among normal Korean adults","volume":"6","author":"Baik SC","year":"1990","journal-title":"J Korean Soc Microbiol"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/168.1.219"},{"key":"e_1_2_6_5_2","first-page":"475","article-title":"Prevalence of Helicobacter pylori infection among normal Koreans","volume":"6","author":"Rhee KH","year":"1990","journal-title":"J Korean Soc Microbiol"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199405053301803"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199104113241507"},{"key":"e_1_2_6_8_2","first-page":"1279","article-title":"Increased oxidative DNA damage Helicobacter pylori\u2010infected human gastric mucosa","volume":"56","author":"Baik SC","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_6_9_2","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/0016-5085(87)91028-6","article-title":"Gastric Campylobacter\u2010like organisms gastritis and peptic ulcer disease","volume":"97","author":"Blaser MJ","year":"1987","journal-title":"Gastroenterology"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.302.6788.1302"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199110173251604"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.316.7143.1507"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.epirev.a036078"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.251551698"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01140.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI12672"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001099900067"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-11-2925"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.61.12.5008-5012.1993"},{"key":"e_1_2_6_20_2","doi-asserted-by":"crossref","first-page":"14648","DOI":"10.1073\/pnas.93.25.14648","article-title":"cag pathogenicity island of Helicobacter pylori encodes type I\u2010specific and disease\u2010associated virulence factors","volume":"93","author":"Censini S","year":"1996","journal-title":"Proc Natl Acad Sci U S A"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2002.05977.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11450"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.5.2346-2348.1998"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000959200"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.2.665-671.2002"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.60.5.1858-1863.1992"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.7.2445-2448.1996"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.174.8.2539-2547.1992"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3923-3929.2002"},{"key":"e_1_2_6_30_2","first-page":"176","article-title":"Genomic\u2010sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori","volume":"14","author":"Alm RA","journal-title":"Nature"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/41483"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150180804"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000401)21:6<1187::AID-ELPS1187>3.0.CO;2-F"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200204)23:7\/8<1161::AID-ELPS1161>3.0.CO;2-7"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01896.x"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1080\/00313020126309"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-5378.2002.00078.x"},{"key":"e_1_2_6_38_2","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1128\/CDLI.7.2.301-306.2000","article-title":"Clustering of clinical strains of Helicobacter pylori analyzed by two\u2010dimensional gel electrophoresis","volume":"7","author":"Enroth H","year":"2000","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11557"},{"key":"e_1_2_6_40_2","first-page":"194","article-title":"Serological responses of FldA and small\u2010molecular\u2010weight proteins of Helicobacter pylori: correlation with the presence of the gastric MALT tissue","volume":"9","author":"Yang HB","journal-title":"Helicobacter 2004"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(99)70553-6"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001099900067"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365529509096306"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-199612000-00003"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018818431828"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005460130305"},{"key":"e_1_2_6_47_2","first-page":"9","article-title":"A prospective study on the Campylobacter pylori isolated from patients of gastroduodenal inflammatory conditions","volume":"23","author":"Rhee KH","year":"1988","journal-title":"J Korean Soc Microbiol"},{"key":"e_1_2_6_48_2","first-page":"543","article-title":"Antibiotic susceptibility of Helicobacter pylori and the combination effect of antibiotics on the antibiotic\u2010resistant H. pylori strains","volume":"34","author":"Song GY","year":"1999","journal-title":"J Korean Soc Microbiol"},{"key":"e_1_2_6_49_2","first-page":"97","article-title":"Two\u2010dimensional gel electrophoresis of Helicobater pylori for proteomic analysis","volume":"35","author":"Jung TS","year":"2000","journal-title":"J Korean Soc Microbiol"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19990301)20:3<601::AID-ELPS601>3.0.CO;2-6"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150180309"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/18.2.298"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300739"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.18.4962-4970.2002"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200202)2:2<164::AID-PROT164>3.0.CO;2-U"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr049758y"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401003"},{"key":"e_1_2_6_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)01115-X"},{"key":"e_1_2_6_59_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200405979"},{"key":"e_1_2_6_60_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200500655"},{"key":"e_1_2_6_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.6.2331-2335.1996"},{"key":"e_1_2_6_62_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(20000701)21:13<2670::AID-ELPS2670>3.0.CO;2-5"},{"key":"e_1_2_6_63_2","doi-asserted-by":"publisher","DOI":"10.1086\/315173"},{"key":"e_1_2_6_64_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.26.14668"},{"key":"e_1_2_6_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.8.4818-4822.2005"},{"key":"e_1_2_6_66_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.40.3.297"},{"key":"e_1_2_6_67_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.43.6.752"},{"key":"e_1_2_6_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.35.7.1710-1714.1997"},{"key":"e_1_2_6_69_2","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1128\/jcm.35.6.1344-1347.1997","article-title":"Equally high prevalences of infection with  cagA\u2010positive Helicobacter pylori in Chinese patients with peptic ulcer disease and those with chronic gastritis\u2010associated dyspepsia","volume":"35","author":"Pan ZJ","year":"1997","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_70_2","doi-asserted-by":"publisher","DOI":"10.1080\/003655299750025075"},{"key":"e_1_2_6_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00162-4"},{"key":"e_1_2_6_72_2","doi-asserted-by":"crossref","first-page":"2470","DOI":"10.1128\/iai.59.7.2470-2475.1991","article-title":"Essential role of urease in pathogenesis of gastritis induced by Helicobacter pylori in gnototiotic piglets","volume":"59","author":"Eaton KA","year":"1991","journal-title":"Immun Infect"},{"key":"e_1_2_6_73_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.12.6002"},{"key":"e_1_2_6_74_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2005.00300.x"},{"key":"e_1_2_6_75_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-46-10-825"},{"key":"e_1_2_6_76_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27527-0"},{"key":"e_1_2_6_77_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.1996.22164005.x"},{"key":"e_1_2_6_78_2","doi-asserted-by":"crossref","unstructured":"KrahA MiehlkeS PleissnerKP Zimny\u2010ArndtU KirschC LehnN MeyerTF JungblutPR AebischerT.Identification of candidate antigens for serologic detection ofHelicobacter pylori\u2010infected patients with gastric carcinoma.Int J Cancer2004;108:456\u201363.","DOI":"10.1002\/ijc.11557"},{"key":"e_1_2_6_79_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01382.x"},{"key":"e_1_2_6_80_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.4034-4040.2001"},{"key":"e_1_2_6_81_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.2000.0388"},{"key":"e_1_2_6_82_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.1.378-384.2005"},{"key":"e_1_2_6_83_2","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1128\/JB.180.5.1119-1128.1998","article-title":"Helicobacter pylori porCDAB and oorDABC genes encode distinct pyruvate: flavodoxin and 2\u2010oxoglutarate: acceptor oxidoreductases which mediate electron transport to NADP","volume":"180","author":"Hughes NJ","year":"1998","journal-title":"J Bacteriol"}],"container-title":["Helicobacter"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1523-5378.2006.00456.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1523-5378.2006.00456.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T23:38:52Z","timestamp":1697153932000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1523-5378.2006.00456.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11,2]]},"references-count":82,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,12]]}},"alternative-id":["10.1111\/j.1523-5378.2006.00456.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1523-5378.2006.00456.x","archive":["Portico"],"relation":{},"ISSN":["1083-4389","1523-5378"],"issn-type":[{"value":"1083-4389","type":"print"},{"value":"1523-5378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11,2]]}}}