{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:12:37Z","timestamp":1790860357432,"version":"4.1.0"},"reference-count":66,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,5,4]],"date-time":"2007-05-04T00:00:00Z","timestamp":1178236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Background:\u2002<\/jats:bold>\n                    The\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    protein HorB (encoded by HP0127) is a member of a paralogous family that includes the adhesins BabA, AlpA, AlpB, and HopZ, which contribute to adhesion to gastric epithelial cells. Of the verified\n                    <jats:italic>H.\u00a0pylori<\/jats:italic>\n                    porins, the HorB sequence is most similar to that of HopE, but the function of HorB is unknown. The aim of our study was to investigate the role of HorB in\n                    <jats:italic>H.\u00a0pylori<\/jats:italic>\n                    gastric epithelial cell adhesion.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Materials and methods:\u2002<\/jats:bold>\n                    We disrupted the\n                    <jats:italic>horB<\/jats:italic>\n                    gene in\n                    <jats:italic>H.\u00a0pylori<\/jats:italic>\n                    and measured the adhesion to gastric epithelial cells (AGS cells). We then assessed the effect that HorB disruption had on lipopolysaccharide (LPS) O\u2010chain production and Lewis\n                    <jats:italic>x<\/jats:italic>\n                    and Lewis\n                    <jats:italic>y<\/jats:italic>\n                    antigen expression. A HorB mutant in the mouse\u2010adapted strain\n                    <jats:italic>H.\u00a0pylori<\/jats:italic>\n                    SS1 was created by marker exchange and mouse stomach colonization was quantified. Using reverse transcription polymerase chain reaction, human gastric biopsy material from\n                    <jats:italic>H.\u00a0pylori\u2010<\/jats:italic>\n                    infected patients was then examined for expression of the\n                    <jats:italic>horB<\/jats:italic>\n                    gene.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:\u2002<\/jats:bold>\n                    Disruption of the\n                    <jats:italic>horB<\/jats:italic>\n                    gene reduced\n                    <jats:italic>H.\u00a0pylori<\/jats:italic>\n                    adhesion by more than twofold. Adhesion in the\n                    <jats:italic>horB<\/jats:italic>\n                    knockout strain was restored to wild\u2010type levels by re\u2010introduction of HorB into the chromosome. Disruption of HorB reduced production of LPS O\u2010chains and lowered the level of expression of Lewis\n                    <jats:italic>x<\/jats:italic>\n                    and Lewis\n                    <jats:italic>y<\/jats:italic>\n                    antigens. Insertional mutagenesis of the\n                    <jats:italic>horB<\/jats:italic>\n                    gene in\n                    <jats:italic>H.\u00a0pylori<\/jats:italic>\n                    SS1 reduced mouse stomach colonization threefold. Finally, expression of the\n                    <jats:italic>horB<\/jats:italic>\n                    gene was detected in human gastric biopsy material from\n                    <jats:italic>H.\u00a0pylori\u2010<\/jats:italic>\n                    infected patients.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:\u2002<\/jats:bold>\n                    From these data we conclude that HorB has a role in\n                    <jats:italic>H.\u00a0pylori<\/jats:italic>\n                    adhesion during infection.\n                  <\/jats:p>","DOI":"10.1111\/j.1523-5378.2007.00499.x","type":"journal-article","created":{"date-parts":[[2007,5,9]],"date-time":"2007-05-09T02:02:51Z","timestamp":1178676171000},"page":"200-209","source":"Crossref","is-referenced-by-count":23,"title":["HorB (HP0127) is a Gastric Epithelial Cell Adhesin"],"prefix":"10.1111","volume":"12","author":[{"given":"William J.","family":"Snelling","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony P.","family":"Moran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kieran A.","family":"Ryan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Scully","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kieran","family":"McGourty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakki C.","family":"Cooney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi","family":"Annuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul W.","family":"O'Toole","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,5,4]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-115-4-266"},{"key":"e_1_2_6_3_2","first-page":"177","article-title":"Helicobacter pylori infection as a cause of gastritis, duodenal ulcer, gastric cancer and nonulcer dyspepsia: a systematic overview","volume":"150","author":"Veldhuyzen van Zanten SJ","year":"1994","journal-title":"Can Med Assoc J"},{"key":"e_1_2_6_4_2","first-page":"1360","article-title":"An international association between Helicobacter pylori infection and gastric cancer","volume":"341","author":"Eurogast SG.","year":"1993","journal-title":"Lancet"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199405053301803"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2002.0160s1003.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(03)00111-4"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-5378.2003.00162.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2003.022111"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(03)00125-4"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555818005.ch34"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/41483"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v11.i15.2260"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8018146"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5349.373"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1069076"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4155-4168.2000"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01300.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.130079797"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.16.3325"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01823.x"},{"key":"e_1_2_6_22_2","first-page":"787","article-title":"Helicobacter pylori lipopolysaccharide\u2010mediated gastric and extragastric pathology","volume":"50","author":"Moran AP.","year":"1999","journal-title":"J Physiol Pharmacol"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00205"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.6.3073-3079.2002"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2876-2880.2003"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(97)01035-4"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.5.2060-2070.1999"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-5378.1999.09046.x"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.4.1567-1572.1995"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.9.2370-2375.2000"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70155-0"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.8.4668-4679.2004"},{"key":"e_1_2_6_33_2","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J","year":"2001"},{"key":"e_1_2_6_34_2","first-page":"15","article-title":"Cloning of Campylobacter jejuni genes required for leucine biosynthesis, and construction of leu\u2010negative mutant of C. jejuni by shuttle transposon mutagenesis","volume":"143","author":"Labigne A","year":"1992","journal-title":"Res Microbiol Inst Pasteur"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.14.3948-3954.2000"},{"key":"e_1_2_6_36_2","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J","year":"1989"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb01307.x"},{"key":"e_1_2_6_38_2","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications","volume":"74","author":"Towbin H","year":"1979","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.2108-2120.2002"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/13.Supplement_8.S657"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2000.tb01506.x"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.2358-2368.2004"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.154.1.269-277.1983"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.4.1370-1377.1992"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90673-X"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09264.x"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2000.tb01532.x"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1999.tb01268.x"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.11.4598-4605.1996"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108574200"},{"key":"e_1_2_6_51_2","first-page":"259","article-title":"Colonization and immune responses in mice to Helicobacter pylori expressing different Lewis antigens","volume":"3","author":"Suresh MR","year":"2000","journal-title":"J Pharm Pharmaceut Sci"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7832-7838.2001"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.10.5877-5881.2002"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1385\/0-89603-381-3:235"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1067\/mpr.2000.108600"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.1997.00164.x"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3529-3538.2002"},{"key":"e_1_2_6_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.10.4526-4533.1994"},{"key":"e_1_2_6_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01377-6"},{"key":"e_1_2_6_60_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1995.tb00043.x"},{"key":"e_1_2_6_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.10.6048-6053.1990"},{"key":"e_1_2_6_62_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00746.x"},{"key":"e_1_2_6_63_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02094.x"},{"key":"e_1_2_6_64_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb01032.x"},{"key":"e_1_2_6_65_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01276.x"},{"key":"e_1_2_6_66_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-8-2285"},{"key":"e_1_2_6_67_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.36589"}],"container-title":["Helicobacter"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1523-5378.2007.00499.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1523-5378.2007.00499.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T14:31:16Z","timestamp":1697121076000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1523-5378.2007.00499.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,4]]},"references-count":66,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,6]]}},"alternative-id":["10.1111\/j.1523-5378.2007.00499.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1523-5378.2007.00499.x","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1084854.537788","asserted-by":"object"}]},"ISSN":["1083-4389","1523-5378"],"issn-type":[{"value":"1083-4389","type":"print"},{"value":"1523-5378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5,4]]}}}