{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:27:06Z","timestamp":1791916026227,"version":"4.3.4"},"reference-count":11,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,3,20]],"date-time":"2012-03-20T00:00:00Z","timestamp":1332201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2012,6]]},"abstract":"Abstract<\/jats:title>Background:\u2002<\/jats:bold> The detection of the putative disease\u2010specific Helicobacter pylori<\/jats:italic> marker duodenal ulcer promoting gene A (dup<\/jats:italic>A) is currently based on PCR detection of jhp0917<\/jats:italic> and jhp0918<\/jats:italic> that form the gene. However, mutations that lead to premature stop codons that split off the dup<\/jats:italic>A leading to truncated products cannot be evaluated by PCR.<\/jats:p>Methods:\u2002<\/jats:bold> We directly sequence the complete dup<\/jats:italic>A of 75 dup<\/jats:italic>A\u2010positive strains of H.\u00a0pylori<\/jats:italic> isolated from patients with gastritis (n\u2003=\u200326), duodenal ulcer (n\u2003=\u200329), and gastric carcinoma (n\u2003=\u200320), to search for frame\u2010shifting mutations that lead to stop codon.<\/jats:p>Results:\u2002<\/jats:bold> Thirty\u2010four strains had single nucleotide mutations in dup<\/jats:italic>A that lead to premature stop codon creating smaller products than the predicted 1839\u2003bp product and, for this reason, were considered as dup<\/jats:italic>A\u2010negative. Intact dup<\/jats:italic>A was more frequently observed in strains isolated from duodenal ulcer patients (65.5%) than in patients with gastritis only (46.2%) or with gastric carcinoma (50%). In logistic analysis, the presence of the intact dup<\/jats:italic>A independently associated with duodenal ulcer (OR\u2003=\u20035.06; 95% CI\u2003=\u20031.22\u201320.96, p<\/jats:italic>\u2003=\u2003.02).<\/jats:p>Conclusion:\u2002<\/jats:bold> We propose the primer walking methodology as a simple technique to sequence the gene. When we considered as dup<\/jats:italic>A\u2010positive only those strains that carry dup<\/jats:italic>A gene without premature stop codons, the gene was associated with duodenal ulcer and, therefore, can be used as a marker for this disease in our population.<\/jats:p>","DOI":"10.1111\/j.1523-5378.2011.00933.x","type":"journal-article","created":{"date-parts":[[2012,3,20]],"date-time":"2012-03-20T10:47:55Z","timestamp":1332240475000},"page":"176-180","source":"Crossref","is-referenced-by-count":10,"title":["Single Nucleotide Polymorphisms of Helicobacter pylori dup<\/i>A that Lead to Premature Stop Codons"],"prefix":"10.1111","volume":"17","author":[{"given":"S\u00edlvia B.","family":"Moura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafaella F. A.","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Anacleto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gifone A.","family":"Rocha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreia M. C.","family":"Rocha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dulciene M. M.","family":"Queiroz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,3,20]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.01.009"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2007.00557.x"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/522177"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01737-07"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-8-49"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/653587"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2010.08.019"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1651-1655.2003"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(01)00225-1"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2007.05.006"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.03081.x"}],"container-title":["Helicobacter"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1523-5378.2011.00933.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1523-5378.2011.00933.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T03:17:37Z","timestamp":1698635857000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1523-5378.2011.00933.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,20]]},"references-count":11,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,6]]}},"alternative-id":["10.1111\/j.1523-5378.2011.00933.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1523-5378.2011.00933.x","archive":["Portico"],"relation":{},"ISSN":["1083-4389","1523-5378"],"issn-type":[{"value":"1083-4389","type":"print"},{"value":"1523-5378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,20]]}}}