{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:18:35Z","timestamp":1791199115567,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,3,20]],"date-time":"2012-03-20T00:00:00Z","timestamp":1332201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Backgrounds:\u2002<\/jats:bold> The levofloxacin resistance caused by <jats:italic>gyrA<\/jats:italic> gene mutation is rising rapidly to limit wide application for <jats:italic>Helicobacter pylori<\/jats:italic> eradication. We investigated whether gemifloxacin has a superior antimicrobial activity to levofloxacin against <jats:italic>H.\u00a0pylori.<\/jats:italic><\/jats:p><jats:p><jats:bold>Materials and Methods:\u2002<\/jats:bold> Forty\u2010four consecutive clinical <jats:italic>H.\u00a0pylori<\/jats:italic> isolates with levofloxacin resistance and 80 randomly selected levofloxacin\u2010sensitive controls were tested for gemifloxacin sensitivity by <jats:italic>E<\/jats:italic>\u2010test. The resistance to levofloxacin or gemifloxacin was defined as minimal inhibitory concentration (MIC) &gt;1\u2003mg\/L. The clinical features and GyrA mutation patterns checked by direct sequencing were also analyzed to assess its association with the <jats:italic>H.\u00a0pylori<\/jats:italic> gemifloxacin resistance.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> All levofloxacin\u2010sensitive <jats:italic>H.\u00a0pylori<\/jats:italic> isolates were sensitive to gemifloxacin. Eight strains (18.2%) resistant to levofloxacin could be still sensitive to gemifloxacin. Gemifloxacin achieved a 5\u2010time lower in MIC levels against levofloxacin\u2010resistant isolates. Nearly all levofloxacin\u2010resistant isolates (97.7%, 43\/44) had GyrA mutation at amino acid position 87 or 91. Double mutation sites may play dual roles in quinolone resistance, as N87K plus H57Y or D91N plus V77A mutations showed high\u2010level resistance to both quinolones; whereas D91Y plus A97V or D91N plus A97V mutations showed low level levofloxacin resistance to become sensitive to gemifloxacin. In <jats:italic>H.\u00a0pylori<\/jats:italic> isolates with single N87K, D91Y or D91N mutation, near 20% was gemifloxacin\u2010sensitive and levofloxacin\u2010resistant. The gemifloxacin\u2010resistant rate of <jats:italic>H.\u00a0pylori<\/jats:italic> was higher in patients with gastric ulcer than in those without (<jats:italic>p\u2003<\/jats:italic>&lt;.05).<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Gemifloxacin is superior to levofloxacin in antimicrobial activity against clinical <jats:italic>H.\u00a0pylori<\/jats:italic> isolates, and even overcome some levofloxacin resistance.<\/jats:p>","DOI":"10.1111\/j.1523-5378.2012.00935.x","type":"journal-article","created":{"date-parts":[[2012,3,20]],"date-time":"2012-03-20T11:47:44Z","timestamp":1332244064000},"page":"210-215","source":"Crossref","is-referenced-by-count":25,"title":["Gemifloxacin Can Partially Overcome Quinolone Resistance of <i>H.\u00a0pylori<\/i> with <i>gyrA<\/i> Mutation in Taiwan"],"prefix":"10.1111","volume":"17","author":[{"given":"Wei\u2010Lun","family":"Chang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Yen","family":"Kao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung\u2010Tai","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ay\u2010Huey","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiunn\u2010Jong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiao\u2010Bai","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiu\u2010Chi","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bor\u2010Shyang","family":"Sheu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,3,20]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2007.132514"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2009.192757"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/83.4.864"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-146-8-200704170-00006"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.215350"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2006.02737.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2007.00507.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2009.05829.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki334"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2006.00415.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2006.00436.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.11.007"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2007.03331.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/46.6.1029"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.1.107"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2009.00655.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01131-10"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/46.2.283"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00049-09"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01552-08"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.12.3942-3944.2003"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/47.3.360"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-1009-9"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1159\/000112416"},{"key":"e_1_2_6_26_2","first-page":"925","article-title":"Detection 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