{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:12:50Z","timestamp":1790971970837,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Alcoholism Clin &amp;amp; Exp Res"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Alcoholic and nonalcoholic steatohepatitis are leading causes of liver diseases worldwide. While of different etiology, these share common pathophysiological mechanisms and feature abnormal fat metabolism, inflammation and fibrosis. MicroRNAs (miRNA) are highly conserved noncoding RNAs that control gene expression at the post\u2010transcriptional level either via the degradation of target mRNAs or the inhibition of translation. Each miRNA controls the expression of multiple targets; miRNAs have been linked to regulation of lipid metabolism and inflammation.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> We fed Lieber\u2010DeCarli alcohol or methionine\u2010choline\u2010deficient (MCD) diets to C57Bl6 and analyzed livers for histopathology, cytokines by ELISA, alanine aminotransferase (ALT) by biochemical assay, and microRNA profile by microarray.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Both Lieber\u2010DeCarli and MCD diets lead to development of liver steatosis, liver injury, indicated by increased ALT, and elevated levels of serum TNF\u03b1, suggesting that animal models portray the pathophysiological features of alcoholic and nonalcoholic fatty liver, respectively. We identified that Lieber\u2010deCarli diet up\u2010regulated 1% and down\u2010regulated 1% of known miRNA; MCD diet up\u2010regulated 3% and down\u2010regulated 1% of known miRNA, compared to controls. Of miRNAs that changed expression levels, 5 miRNAs were common in alcoholic and nonalcoholic fatty livers: the expression of both miR\u2010705 and miR\u20101224 was increased after Lieber\u2010DeCarli or MCD diet feeding. In contrast, miR\u2010182, miR\u2010183, and miR\u2010199a\u20103p were down\u2010regulated in Lieber\u2010deCarli feeding, while MCD diet lead to their up\u2010regulation, compared to corresponding controls.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Our findings indicate etiology\u2010specific changes in miRNA expression profile during steatohepatitis models, which opens new avenues for research in the pathophysiology of alcoholic and nonalcoholic fatty liver disease.<\/jats:p>","DOI":"10.1111\/j.1530-0277.2009.01007.x","type":"journal-article","created":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T17:57:15Z","timestamp":1246471035000},"page":"1704-1710","source":"Crossref","is-referenced-by-count":161,"title":["MicroRNA Expression Profile in Lieber\u2010DeCarli Diet\u2010Induced Alcoholic and Methionine Choline Deficient Diet\u2010Induced Nonalcoholic Steatohepatitis Models in Mice"],"prefix":"10.1111","volume":"33","author":[{"given":"Angela","family":"Dolganiuc","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Petrasek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Kodys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna","family":"Catalano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pranoti","family":"Mandrekar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arumugam","family":"Velayudham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyongyi","family":"Szabo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,9,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02871"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-5-29"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161208785294627"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.04.031"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.12706"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2001-12925"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.34168"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20081219"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2008.00630.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2008.080096"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07086"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.05.041"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2002.tb02616.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2006.01.005"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1722"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2008.02.022"},{"key":"e_1_2_5_18_1","first-page":"271","article-title":"Hedgehog signaling, epithelial\u2010to\u2010mesenchymal transition and miRNA","volume":"22","author":"Katoh Y","year":"2008","journal-title":"Int J Mol Med"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04303"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI105200"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20081062"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2008.05.004"},{"key":"e_1_2_5_23_1","first-page":"150","article-title":"Emerging role of microRNAs in disease pathogenesis and strategies for therapeutic modulation","volume":"10","author":"Mattes J","year":"2008","journal-title":"Curr Opin Mol Ther"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.1998.00587.x"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.09.020"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.alc.0000191777.36629.33"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2008.05.032"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21614"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.07.031"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.183426"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.2174\/157016308783769478"},{"key":"e_1_2_5_32_1","unstructured":"Sanger Institute microRNA database. 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