{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T11:40:43Z","timestamp":1725622843676},"reference-count":61,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,3,13]],"date-time":"2012-03-13T00:00:00Z","timestamp":1331596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Alcoholism Clin &amp; Exp Res"],"published-print":{"date-parts":[[2012,8]]},"abstract":"Background<\/jats:title>Individual differences in susceptibility to the detrimental effects of prenatal ethanol (EtOH<\/jats:styled-content>) exposure have been demonstrated. Many factors, including genetics, play a role in susceptibility and resistance. We have previously shown that C<\/jats:styled-content>57BL<\/jats:styled-content>\/6J (B<\/jats:styled-content>6) mice display a number of morphological malformations following an acute dose of EtOH<\/jats:styled-content> in utero, while DBA<\/jats:styled-content>\/2J<\/jats:styled-content> (D<\/jats:styled-content>2) mice are relatively resistant. Here, we present the results of quantitative trait locus (QTL<\/jats:styled-content>) mapping for EtOH<\/jats:styled-content> teratogenesis in recombinant inbred strains derived from a cross between B<\/jats:styled-content>6 and D<\/jats:styled-content>2 (BXD RI<\/jats:styled-content>s).<\/jats:p><\/jats:sec>Methods<\/jats:title>Pregnant dams were intubated with either maltose\u2010dextrin or 5.8\u00a0g\/kg EtOH<\/jats:styled-content> on day 9 of gestation (GD<\/jats:styled-content>9). On GD<\/jats:styled-content> 18, dams were sacrificed and fetuses and placentae were removed. Placentae and fetuses were weighed; fetuses were sexed and examined for gross morphological malformations. Fetuses were then either placed in B<\/jats:styled-content>ouin's fixative for subsequent soft\u2010tissue analyses or eviscerated and placed in EtOH<\/jats:styled-content> for subsequent skeletal examinations. QTL<\/jats:styled-content> mapping for maternal weight gain (MWG<\/jats:styled-content>), prenatal mortality, fetal weight (FW<\/jats:styled-content>) at c\u2010section, placental weight (PW<\/jats:styled-content>), and several morphological malformations was performed using WebQTL<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>Heritability for our traits ranged from 0.06 for PW<\/jats:styled-content> to 0.39 for MWG<\/jats:styled-content>. We found suggestive QTL<\/jats:styled-content>s mediating all phenotypes and significant QTL<\/jats:styled-content>s for FW<\/jats:styled-content> and digit and rib malformations. While most QTL<\/jats:styled-content> regions are large, several intriguing candidate genes emerged based on polymorphisms between B<\/jats:styled-content>6 and D<\/jats:styled-content>2 and gene function.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>In this first mapping study for EtOH<\/jats:styled-content> teratogenesis, several QTL<\/jats:styled-content>s were identified. Future studies will further characterize these regions. Identification of genes and epigenetic modifications mediating susceptibility to the teratogenic effects of alcohol in mice will provide targets to examine in human populations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1530-0277.2012.01754.x","type":"journal-article","created":{"date-parts":[[2012,3,13]],"date-time":"2012-03-13T18:41:24Z","timestamp":1331664084000},"page":"1340-1354","source":"Crossref","is-referenced-by-count":16,"title":["Quantitative Trait Locus Mapping for Ethanol Teratogenesis in BXD<\/scp> Recombinant Inbred Mice"],"prefix":"10.1111","volume":"36","author":[{"given":"Chris","family":"Downing","sequence":"first","affiliation":[{"name":"Institute for Behavioral Genetics University of Colorado Boulder Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Balderrama\u2010Durbin","sequence":"additional","affiliation":[{"name":"Institute for Behavioral Genetics University of Colorado Boulder 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