{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T08:31:28Z","timestamp":1771057888839,"version":"3.50.1"},"reference-count":7,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,4,6]],"date-time":"2012-04-06T00:00:00Z","timestamp":1333670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Alcoholism Clin &amp; Exp Res"],"published-print":{"date-parts":[[2012,10]]},"abstract":"Background<\/jats:title>The biomarkers of excessive alcohol intake reported thus far have not always been reliable. We discovered the presence of free glycerol (FG) by high\u2010performance liquid chromatography (HPLC) in the serum of alcoholic patients but not in healthy persons, and a higher percentage of the alcoholics were positive for serum FG<\/jats:styled-content> than for gamma\u2010glutamyl transpeptidase, mean corpuscular volume, or carbohydrate\u2010deficient transferrin (%CDT). Therefore, in this study, we investigated whether the same results as with HPLC<\/jats:styled-content> could be obtained by measuring FG<\/jats:styled-content> with an easy\u2010to\u2010use autoanalyzer and whether the serum FG<\/jats:styled-content> measured by this method would be useful as a biomarker of excessive alcohol intake.<\/jats:p><\/jats:sec>Methods<\/jats:title>First, the measurements of serum FG<\/jats:styled-content> made by HPLC<\/jats:styled-content> and by a biochemical method with an autoanalyzer were tested for a correlation, and then fasting serum FG<\/jats:styled-content> was measured with the autoanalyzer in 3 groups: a group of Japanese male alcoholics who drank until just before admission, a group of J<\/jats:styled-content>apanese male patients with chronic viral hepatitis, and a group of J<\/jats:styled-content>apanese healthy male volunteers.<\/jats:p><\/jats:sec>Results<\/jats:title>There was a strong positive correlation between the serum FG<\/jats:styled-content> values measured by HPLC<\/jats:styled-content> and by the autoanalyzer in the alcoholic group. The values in the alcoholic group were significantly higher than in the viral hepatitis group and healthy control group. We set the cutoff serum FG<\/jats:styled-content> value for discriminating between the alcoholic group and the other 2 groups in the receiver operating characteristic analysis at 0.9\u00a0mg\/dl, and that cutoff value provided a sensitivity of 80% for identifying the alcoholic group and a specificity of 84 and 94% in relation to the viral hepatitis group and the healthy volunteer group, respectively. Among various clinical tests in the alcoholic group, serum FG<\/jats:styled-content> showed the highest rate of abnormally high value.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Measurement of serum FG<\/jats:styled-content> with an autoanalyzer may be useful as a biomarker of excessive alcohol intake.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1530-0277.2012.01795.x","type":"journal-article","created":{"date-parts":[[2012,4,6]],"date-time":"2012-04-06T18:19:56Z","timestamp":1333736396000},"page":"1820-1826","source":"Crossref","is-referenced-by-count":5,"title":["Higher Serum Free Glycerol Levels in a Group of Alcoholics than in Controls"],"prefix":"10.1111","volume":"36","author":[{"given":"Katsuya","family":"Maruyama","sequence":"first","affiliation":[{"name":"Clinical Research Unit National Hospital Organization Kurihama Alcoholism Center Yokosuka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Clinical Research Unit National Hospital Organization Kurihama Alcoholism Center Yokosuka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshifumi","family":"Matsui","sequence":"additional","affiliation":[{"name":"Clinical Research Unit National Hospital Organization Kurihama Alcoholism Center Yokosuka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Mizukami","sequence":"additional","affiliation":[{"name":"Clinical Research Unit National Hospital Organization Kurihama Alcoholism Center Yokosuka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Mizukami","sequence":"additional","affiliation":[{"name":"Clinical Research Unit National Hospital Organization Kurihama Alcoholism Center Yokosuka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyuki","family":"Sogawa","sequence":"additional","affiliation":[{"name":"Clinical Proteomics Research Center Chiba University Hospital Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Yokosuka","sequence":"additional","affiliation":[{"name":"Department of Medicine and Clinical Oncology Graduate School of Medicine Chiba University Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumio","family":"Nomura","sequence":"additional","affiliation":[{"name":"Clinical Proteomics Research Center Chiba University Hospital Chiba Japan"},{"name":"Departments of Clinical Laboratory and Molecular Diagnosis Chiba University Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuji","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Department of Human Resources Development National Institute of Public Health Wako Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,4,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2002.tb02658.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2002.tb02542.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.190"},{"key":"e_1_2_7_5_1","first-page":"33","article-title":"Comparison of free\u2010glycerol with 5.9 kD peptide as a serum biomarker of excessive alcohol intake","volume":"28","author":"Maruyama K","year":"2009","journal-title":"Alcohol Biomed Res"},{"key":"e_1_2_7_6_1","unstructured":"MizukamiY HozakiK OkazakiM MizunoT MaruyamaK(2002)Effects of alcohol consumption on serum lipids and lipoproteins(in Japanese). Bulletin of Jissen Women's University Faculty of Human Life Sciences 39:33\u201339."},{"key":"e_1_2_7_7_1","first-page":"110","article-title":"Analysis of lipoprotein metabolism in alcoholics","volume":"43","author":"Mizukami Y","year":"2008","journal-title":"Jpn J Alcohol & Drug Dependence"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2006.00282.x"}],"container-title":["Alcoholism: Clinical and Experimental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1530-0277.2012.01795.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1530-0277.2012.01795.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T00:57:41Z","timestamp":1695517061000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1530-0277.2012.01795.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4,6]]},"references-count":7,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2012,10]]}},"alternative-id":["10.1111\/j.1530-0277.2012.01795.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1530-0277.2012.01795.x","archive":["Portico"],"relation":{},"ISSN":["0145-6008","1530-0277"],"issn-type":[{"value":"0145-6008","type":"print"},{"value":"1530-0277","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4,6]]}}}