{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T08:10:46Z","timestamp":1765354246971,"version":"3.38.0"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,7,19]],"date-time":"2011-07-19T00:00:00Z","timestamp":1311033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Surgery"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:p><jats:bold>Objective:<\/jats:bold>To evaluate lymphocyte populations in stifle synovium and synovial fluid of dogs with degenerative cranial cruciate ligament rupture (CCLR).<\/jats:p><jats:p><jats:bold>Study Design:<\/jats:bold>Prospective clinical study.<\/jats:p><jats:p><jats:bold>Animals:<\/jats:bold>Dogs (n=25) with stifle arthritis and CCLR, 7 dogs with arthritis associated with cartilage degeneration (osteoarthritis [OA]), and 12 healthy Beagle dogs with intact CCL.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold>Arthritis was graded radiographically in CCLR dogs. After collection of joint tissues, mononuclear cells were isolated and subsequently analyzed using flow cytometry for expression of CD3, CD4, CD8, and CD21.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold>The proportions of CD4<jats:sup>+<\/jats:sup>T helper lymphocytes, CD8<jats:sup>+<\/jats:sup>cytotoxic T lymphocytes, and CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>\u2212<\/jats:sup>CD8<jats:sup>\u2212<\/jats:sup>T lymphocytes were increased in synovium from dogs with CCLR compared with synovium from healthy Beagle dogs (<jats:italic>P<\/jats:italic>&lt;.05). The proportion of CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>\u2212<\/jats:sup>CD8<jats:sup>\u2212<\/jats:sup>T lymphocytes in synovial fluid was increased in dogs with CCLR compared with dogs with OA (<jats:italic>P<\/jats:italic>&lt;.05). In dogs with CCLR, the proportion of CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>\u2212<\/jats:sup>CD8<jats:sup>\u2212<\/jats:sup>T lymphocytes in synovial fluid was inversely correlated with radiographic arthritis (S<jats:sub>R<\/jats:sub>=\u22120.68,<jats:italic>P<\/jats:italic>&lt;.005).<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold>Lymphocytic inflammation of stifle synovium and synovial fluid is an important feature of the CCLR arthropathy. Lymphocyte populations include T lymphocytes expressing CD4 and CD8, and CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>\u2212<\/jats:sup>CD8<jats:sup>\u2212<\/jats:sup>T lymphocytes. Presence of CD3<jats:sup>+<\/jats:sup>CD4<jats:sup>\u2212<\/jats:sup>CD8<jats:sup>\u2212<\/jats:sup>T lymphocytes was associated with development of stifle synovitis. Further work is needed to fully identify the phenotype of these cells.<\/jats:p>","DOI":"10.1111\/j.1532-950x.2011.00867.x","type":"journal-article","created":{"date-parts":[[2011,7,19]],"date-time":"2011-07-19T23:21:38Z","timestamp":1311117698000},"page":"753-761","source":"Crossref","is-referenced-by-count":27,"title":["Lymphocyte Populations in Joint Tissues from Dogs with Inflammatory Stifle Arthritis and Associated Degenerative Cranial Cruciate Ligament Rupture"],"prefix":"10.1111","volume":"40","author":[{"given":"Peter","family":"Muir","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer L.","family":"Kelly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah Jane","family":"Marvel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel A.","family":"Heinrich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan L.","family":"Schaefer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul A.","family":"Manley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kavita","family":"Tewari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anju","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Suresh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengling","family":"Hao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erin","family":"Plisch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,7,19]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2005.227.1604"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.1988.tb02286.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.2460\/ajvr.1985.46.05.1166","article-title":"Synovial membrane changes after experimental transection of the cranial cruciate ligament in dogs","volume":"46","author":"Lipowitz AJ","year":"1985","journal-title":"Am J Vet Res"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780330913"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2004.07.002"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.68.3.283"},{"key":"e_1_2_6_8_2","article-title":"Synovitis in dogs with stable stifle joints and incipient cranial cruciate ligament rupture","author":"Bleedorn JA","year":"2011","journal-title":"a cross-sectional study"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-198264060-00014"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20183"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1532-950X.2005.00036.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2005.66.493"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1532-950X.2005.00073.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2005.03.012"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1053\/jvet.2003.50023"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2003.64.1010"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2005.66.2073"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2005.09.001"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.69.5.625"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(98)00173-1"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2007.05.016"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.05.023"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2003.09.007"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.2754\/avb200473010079"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.3109\/03009749009102120"},{"key":"e_1_2_6_26_2","first-page":"143","article-title":"Radiographic progression of osteoarthritis of the canine stifle joint","volume":"45","author":"Innes JF","year":"2004","journal-title":"a prospective study"},{"key":"e_1_2_6_27_2","first-page":"31","article-title":"Studies of canine leucocyte antigens","volume":"153","author":"Williams DL","year":"1997","journal-title":"a significant advance in canine immunology"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.9.3849"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.62.3.196"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.5326\/15473317-31-4-289"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.235.4.386"},{"key":"e_1_2_6_32_2","first-page":"239","article-title":"Lymphatic organ development in dogs","volume":"104","author":"Faldyna M","year":"2005","journal-title":"major lymphocyte subsets and activity"},{"key":"e_1_2_6_33_2","first-page":"411","article-title":"Gamma\/delta T\u2010cell lymphoma in a dog","volume":"50","author":"Borska P","year":"2009","journal-title":"Can Vet J"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.4.1277"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200007063430107"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(99)00051-3"},{"key":"e_1_2_6_37_2","first-page":"569","article-title":"Canine epitheliotropic cutaneous T\u2010cell lymphoma","volume":"20","author":"Moore PF","year":"2009","journal-title":"an investigation of T-cell receptor immunophenotype, lesion topography and molecular clonality"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002510100372"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1267"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(00)00230-0"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.24687"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2009.08.002"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2009.11.007"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(96)05563-8"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(98)00229-3"},{"key":"e_1_2_6_46_2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1055\/s-0038-1632931","article-title":"Cytokine profile in canine immune\u2010mediated polyarthritis and osteoarthritis","volume":"18","author":"Hegemann N","year":"2005","journal-title":"Vet Comp Orthop Traumat"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780300311"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.2007.00977.x"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2006.10.002"},{"key":"e_1_2_6_50_2","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.2460\/javma.1993.203.07.1016","article-title":"Epidemiology of cranial cruciate ligament rupture in dogs","volume":"203","author":"Whitehair JG","year":"1993","journal-title":"J Am Vet Med Assoc"}],"container-title":["Veterinary Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1532-950X.2011.00867.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1532-950X.2011.00867.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T05:57:02Z","timestamp":1741327022000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1532-950X.2011.00867.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7,19]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2011,8]]}},"alternative-id":["10.1111\/j.1532-950X.2011.00867.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1532-950x.2011.00867.x","archive":["Portico"],"relation":{},"ISSN":["0161-3499","1532-950X"],"issn-type":[{"type":"print","value":"0161-3499"},{"type":"electronic","value":"1532-950X"}],"subject":[],"published":{"date-parts":[[2011,7,19]]}}}