{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:48:46Z","timestamp":1761540526550},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,5,24]],"date-time":"2004-05-24T00:00:00Z","timestamp":1085356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:p><jats:bold>BACKGROUND:\u2002<\/jats:bold> PLTs stored at 22\u00b0C have the potential for bacterial contamination, a problem that could be reduced by 4\u00b0C storage. Nevertheless, PLTs stored at 4\u00b0C exhibit a significantly reduced life span. This study was performed to determine whether treatment of PLTs with thrombopoietin or cytochalasin B plus ethylene glycol bis(\u03b2\u2010aminoethyl ether) N,N\u2032\u2010tetraacetic acid (EGTA)\u2010AM could prevent exponential loss of PLTs stored at 4\u00b0C.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:\u2002<\/jats:bold> Autologous baboon PLTs were stored at 22 or 4\u00b0C. The 4\u00b0C stored PLTs were treated with 1.5 ng per mL thrombopoietin, with 1 \u00b5mol per L cytochalasin B, and 80 \u00b5mol per L EGTA\u2010AM (cyto\u2010EGTA) or not treated and labeled with <jats:sup>111<\/jats:sup>In\u2010oxine to study their in vivo recovery and life span. PLT function was assessed by correction of an aspirin\u2010induced prolonged bleeding time. Aggregation responses and morphology were also assessed.<\/jats:p><jats:p><jats:bold>RESULTS:\u2002<\/jats:bold> PLTs stored at 22\u00b0C had normal in vivo recovery and linear survival. PLTs stored at 4\u00b0C, whether or not they were treated with thrombopoietin, had normal recovery and exponential survival. Aggregation of cyto\u2010EGTA\u2010treated PLTs was similar for PLTs stored at 4\u00b0C and fresh PLTs, but decreased in PLTs stored at 22\u00b0C for 5 days. The addition of cyto\u2010EGTA to PLTs before 4\u00b0C storage inhibited morphologic changes that occurred in PLTs stored at 22\u00b0C and cold\u2010induced PLT clumping, but did not prevent exponential disappearance of the PLTs.<\/jats:p><jats:p><jats:bold>CONCLUSION:\u2002<\/jats:bold> Addition of thrombopoietin or cyto\u2010chalasin B and EGTA\u2010AM to PLTs before 4\u00b0C storage did not prevent exponential loss of PLTs.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2004.03326.x","type":"journal-article","created":{"date-parts":[[2004,5,24]],"date-time":"2004-05-24T08:51:28Z","timestamp":1085388688000},"page":"865-870","source":"Crossref","is-referenced-by-count":15,"title":["Effect of thrombopoietin alone and a combination of cytochalasin B and ethylene glycol bis(\u03b2\u2010aminoethyl ether) N,N\u2032\u2010tetraacetic acid\u2010AM on the survival and function of autologous baboon platelets stored at 4\u00b0C for as long as 5 days"],"prefix":"10.1111","volume":"44","author":[{"given":"C. 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