{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:33:30Z","timestamp":1771461210470,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2004,7,20]],"date-time":"2004-07-20T00:00:00Z","timestamp":1090281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:p><jats:bold>BACKGROUND:\u2002<\/jats:bold> A photochemical treatment (PCT) process utilizing amotosalen hydrochloride and long wavelength UVA light has been developed to inactivate pathogens in PLTs. This study investigated the effects of amotosalen\/UVA treatment on free and latent murine CMV (MCMV) in PLT preparations using a murine model of transfusion\u2010transmitted CMV (TT\u2010CMV).<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:\u2002<\/jats:bold> In a model of latent MCMV infection, \u201cdonor\u201d mice received 1 \u00d7 10<jats:sup>6<\/jats:sup> plaque\u2010forming units (PFUs) MCMV and were rested 14 days. Subsequently harvested, pooled, and washed WBCs were PCR positive for MCMV. Murine WBC doses of 1 \u00d7 10<jats:sup>4<\/jats:sup>, 1 \u00d7 10<jats:sup>5<\/jats:sup>, and 1 \u00d7 10<jats:sup>6<\/jats:sup> were added to human apheresis PLTs in 35 percent autologous plasma and 65 percent PLT AS (PAS). The WBC\u2010PLT products were treated with 150\u2003\u00b5mol\/L amotosalen and 0.6\u2003J per cm<jats:sup>2<\/jats:sup> UVA and transfused via tail vein injection into recipient mice. Recipients were killed on Day 14. Blood and spleens were collected and assayed for MCMV by PCR. In a parallel model of active infection with free virus, human PLT in 35 percent autologous plasma and 65 percent PAS were dosed with 1 \u00d7 10<jats:sup>5<\/jats:sup> and 1 \u00d7 10<jats:sup>6<\/jats:sup> PFUs of MCMV. All other procedures were as described above.<\/jats:p><jats:p><jats:bold>RESULTS:\u2002<\/jats:bold> In the absence of amotosalen\/UVA\u2010pretreatment, transfusion of PLT latently or actively infected with MCMV produced TT\u2010CMV in a dose\u2010dependent fashion. In contrast, all transfusion recipients of identical PLT preparations pretreated with amotosalen\/UVA were uniformly PCR negative for MCMV (abrogation of TT\u2010CMV; p &lt; 0.05).<\/jats:p><jats:p><jats:bold>CONCLUSIONS:\u2002<\/jats:bold> PCT of PLT preparations with the specified doses of amotosalen hydrochloride and UVA light prevents transfusion transmission of free and latent MCMV in a murine model. These results suggest that PCT of human PLTs with amotosalen\/UVA should also effectively abrogate TT\u2010CMV in the clinical setting.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2004.03351.x","type":"journal-article","created":{"date-parts":[[2004,7,20]],"date-time":"2004-07-20T11:58:25Z","timestamp":1090324705000},"page":"1159-1165","source":"Crossref","is-referenced-by-count":17,"title":["Photochemical treatment of platelet concentrates with amotosalen hydrochloride and ultraviolet A light inactivates free and latent cytomegalovirus in a murine transfusion model"],"prefix":"10.1111","volume":"44","author":[{"given":"Catherine T.","family":"Jordan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natia","family":"Saakadze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James L.","family":"Newman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Levan J.","family":"Lezhava","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanya T.","family":"Maiers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Whitney M.","family":"Hillyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D.","family":"Roback","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher D.","family":"Hillyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,7,20]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1986.tb01990.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/12.Supplement_7.S711"},{"key":"e_1_2_6_4_2","first-page":"211","volume":"12","author":"Roback JD. CMV","year":"2002","journal-title":"Virology"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1994.341095026982.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1992.32392213798.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1986.26386209390.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.18.6.1296-1300.1983"},{"key":"e_1_2_6_9_2","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1128\/jcm.21.3.328-331.1985","article-title":"Comparison of a latex agglutination test with five other methods for determining the presence of antibody against cytomegalovirus","volume":"21","author":"Beckwith DG","year":"1985","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_10_2","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1128\/jcm.22.1.68-70.1985","article-title":"Detection of cytomegalovirus antibody with latex agglutination","volume":"22","author":"Adler SP","year":"1985","journal-title":"J Clin Microbiol"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.22.6.1014-1019.1985"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1992.32792391032.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/171.4.1002"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41101249.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2003.00312.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1987.27688071699.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/154.1.169"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/157.3.523"},{"key":"e_1_2_6_19_2","first-page":"227","article-title":"Prevention of cytomegalovirus infection following bone marrow transplantation: a randomized trial of blood product screening","volume":"7","author":"Miller WJ","year":"1991","journal-title":"Bone Marrow Transplant"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.9.3598.bloodjournal8693598"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2003.00337.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1309\/PVFR-DDWE-302T-WFA1"},{"key":"e_1_2_6_23_2","first-page":"1137","volume":"103","author":"Visconti MR","year":"2004","journal-title":"Assessment of removal of human cytomegalovirus from blood components by leukocyte depletion filters using real-time quantitative PCR"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-10-3143"},{"key":"e_1_2_6_25_2","first-page":"549","article-title":"New technologies for the inactivation of infectious pathogens in cellular blood components and the development of platelet substitutes","volume":"13","author":"Corash L.","year":"2000","journal-title":"Bailli\u00e8re's Clin Haematol"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1997.37497265344.x"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0037-1963(01)90121-0"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1999.39399219279.x"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2000.7940206.x"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.2.475-479.2003"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2000.7840209.x"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00220.x"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1998.tb05418.x"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-03-0932"},{"key":"e_1_2_6_35_2","first-page":"2S","article-title":"Transfusion\u2010transmitted cytomegalovirus infection (TT\u2010CMV): influence of leukoreduction (LR) and allogeneic interactions in a murine model","volume":"39","author":"Roback JD","year":"1999","journal-title":"Transfusion"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/135.5.841"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.67.9.5360-5366.1993"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.11.6023"},{"key":"e_1_2_6_39_2","first-page":"33","volume-title":"Payment","author":"Payment P","year":"1993"},{"key":"e_1_2_6_40_2","doi-asserted-by":"crossref","unstructured":"SlobedmanB MocarskiES.Quantitative analysis of latent human cytomegalovirus. J Virol1999;73:4806\u201012.","DOI":"10.1128\/JVI.73.6.4806-4812.1999"},{"key":"e_1_2_6_41_2","doi-asserted-by":"crossref","unstructured":"ZanghelliniF BoppanaSB EmeryVC et al.Asymptomatic primary cytomegalovirus infection: virologic and immunologic features. J Infect Dis1999;180:702\u20107.","DOI":"10.1086\/314939"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.11.3640"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2000.40050500.x"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1537-2995.2004.03351.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1537-2995.2004.03351.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1537-2995.2004.03351.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T13:30:16Z","timestamp":1697290216000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1537-2995.2004.03351.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,7,20]]},"references-count":42,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2004,8]]}},"alternative-id":["10.1111\/j.1537-2995.2004.03351.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1537-2995.2004.03351.x","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,7,20]]}}}