{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T18:14:12Z","timestamp":1791137652901,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,10,10]],"date-time":"2005-10-10T00:00:00Z","timestamp":1128902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Two recent probable cases of transmission of a variant of human Creutzfeldt\u2010Jakob disease (vCJD) through blood transfusion suggest that the disease can be transmitted through transfusion of blood components from presymptomatic blood donors. In the absence of a preclinical screening test, removal of the infectious agent by processing is the only means by which risk to recipients of blood from donors with inapparent vCJD infections can be eliminated.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold> In the endogenous infectivity study, a pool of 500\u2003mL of whole blood was collected into CP2D anticoagulant from 263K\u2010strain scrapie\u2010infected hamsters, processed into 300\u2003mL of red cells (RBCs), and then passed through a prion removal filter. Pre\u2010 and postfiltration samples were tested for PrP<jats:sup>sc<\/jats:sup> by Western blot and for infectivity by inoculation of healthy hamsters. In the exogenous (spiking) infectivity study, 30\u2003mL of 10\u2003percent (wt\/vol) scrapie\u2010infected brain homogenates was added to 270\u2003mL of human RBCs and then filtered. Levels of PrP<jats:sup>sc<\/jats:sup> and infectivity were determined by Western blot and bioassay.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> In the endogenous infectivity study, the prefiltered RBCs transmitted disease to 6 of 43 animals, whereas the postfiltered RBCs did not transmit disease to any of 35 animals, and a barely visible prefiltration PrP<jats:sup>sc<\/jats:sup> Western blot signal was reduced below the level of detection in the postfiltration sample. In the exogenous (spike) study, infectivity was reduced by 3.7\u2003log LD<jats:sub>50<\/jats:sub> per mL, from 9.2 to 5.5 log LD<jats:sub>50<\/jats:sub> per mL.<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold> The new filter was effective in removing both infectivity and PrP<jats:sup>sc<\/jats:sup> from RBCs. The use of this type of filter should reduce the risk of vCJD transmission through blood transfusion.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2005.00640.x","type":"journal-article","created":{"date-parts":[[2005,10,10]],"date-time":"2005-10-10T10:04:44Z","timestamp":1128938684000},"page":"1839-1844","source":"Crossref","is-referenced-by-count":30,"title":["Removal of exogenous (spiked) and endogenous prion infectivity from red cells with a new prototype of leukoreduction 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