{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:39:38Z","timestamp":1761154778991},"reference-count":14,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,4,6]],"date-time":"2005-04-06T00:00:00Z","timestamp":1112745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> The aim was to evaluate platelet concentrates (PCs) prepared by the automated OrbiSac system, from pooled buffy coats (BCs) stored in a platelet (PLT) additive solution.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold> Experiment\u20031 was a paired in vitro study of PCs (from six\u2003B<jats:sc>Cs<\/jats:sc>), prepared by automated and manual procedures. Experiments\u20032 and 3 evaluated PCs from OrbiSac (from six\u2003B<jats:sc>Cs<\/jats:sc>); Experiment\u20033 included selection of BCs based on donor data. Experiment\u20034 was a paired in vitro study of PCs (from six\u2003B<jats:sc>Cs<\/jats:sc>) with an integrated white blood cell (WBC) filter and two different storage containers. Experiment 5 evaluated PCs (from six\u2003B<jats:sc>Cs<\/jats:sc>) from the OrbiSac with an integrated WBC filter. Experiment\u20036 was similar to Experiment\u20035 with computer\u2010selected pools of 5\u2003B<jats:sc>Cs<\/jats:sc>. The in vitro studies evaluated the effects of 7\u2010day storage of PLTs regarding PLT metabolism and disintegration.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> Experiments\u20031 and 4 had similar in vitro results. In Experiment\u20032, PLT content was 370\u2003\u00d7\u200310<jats:sup>9<\/jats:sup>\u2003\u00b1 70\u2003\u00d7\u200310<jats:sup>9<\/jats:sup> per PC and recovery from BCs was 76\u2003\u00b1\u20036 percent. In Experiment\u20033, the PLT content was 380\u2003\u00d7\u200310<jats:sup>9<\/jats:sup>\u2003\u00b1\u200350\u2003\u00d7\u200310<jats:sup>9<\/jats:sup> per PC and variation was reduced compared with randomly pooled BCs. In Experiment\u20035, increased PLT content was found (420\u2003\u00d7\u200310<jats:sup>9<\/jats:sup>\u2003\u00b1\u200370\u2003\u00d7\u200310<jats:sup>9<\/jats:sup> per PC and recovery from BCs of 80\u2003\u00b1\u20035%). In Experiment\u20036, five rather than six\u2003B<jats:sc>Cs<\/jats:sc> gave 340\u2003\u00d7\u200310<jats:sup>9<\/jats:sup>\u2003\u00b1 60\u2003\u00d7\u200310<jats:sup>9<\/jats:sup> PLTs per PC and recovery was 79\u2003\u00b1\u20035\u2003percent.<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold> These in vitro studies suggest that the OrbiSac technique is equivalent to the standard manual method regarding the PLT in vitro characteristics during storage for 7\u2003days. The results of standardizing the PLT count in PCs by selecting the BCs pools on the basis of the blood donor PLT concentration were encouraging.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2005.04096.x","type":"journal-article","created":{"date-parts":[[2005,4,21]],"date-time":"2005-04-21T23:18:22Z","timestamp":1114125502000},"page":"743-751","source":"Crossref","is-referenced-by-count":37,"title":["Automated preparation of platelet concentrates from pooled buffy coats: in vitro studies and experiences with the OrbiSac system"],"prefix":"10.1111","volume":"45","author":[{"given":"S.","family":"Larsson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Sandgren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Sj\u00f6din","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Vesterinen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Gulliksson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,6]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0955-3886(94)90086-8"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0955-3886(94)90167-8"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-7963(99)80029-7"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3148.2000.00262.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1182\/blood.V46.2.209.209","article-title":"Platelet storage at 22\u00b0: role of gas transport across plastic containers in maintenance of viability","volume":"46","author":"Murphy S","year":"1975","journal-title":"Blood"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1983.tb01918.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1990.tb02053.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1996.36896374382.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2000.7830176.x"},{"key":"e_1_2_6_11_2","first-page":"437","article-title":"Leakage of adenylate kinase from stored blood cells","volume":"5","author":"Olsson T","year":"1983","journal-title":"J Appl Biochem"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1998.38198141495.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1988.tb04699.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1053\/tm.1997.0110116"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-3886(96)00073-2"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1537-2995.2005.04096.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1537-2995.2005.04096.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T11:19:01Z","timestamp":1697282341000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1537-2995.2005.04096.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4,6]]},"references-count":14,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2005,5]]}},"alternative-id":["10.1111\/j.1537-2995.2005.04096.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1537-2995.2005.04096.x","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,4,6]]}}}