{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:00:00Z","timestamp":1792699200339,"version":"4.5.0"},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,1,20]],"date-time":"2006-01-20T00:00:00Z","timestamp":1137715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:p><jats:bold>BACKGROUND:\u2002<\/jats:bold> Red cell (RBC) depletion is needed to bypass ABO mismatch in allogeneic bone marrow transplantation (BMT). Technical and clinical data obtained after bone marrow (BM) processing with a continuous\u2010flow cell separator (Cobe Spectra, Gambro BCT) are reported.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:\u2002<\/jats:bold> RBC depletion and recovery of nucleated cells, CD3+ cells, CD34+ cells, and colony\u2010forming unit\u2013granulocyte\u2010macrophage were calculated. Bacteriologic contaminations, side effects of graft infusion, and hematopoietic recovery were analyzed.<\/jats:p><jats:p><jats:bold>RESULTS:\u2002<\/jats:bold> A total of 114 BM samples were processed. The mean volume collected was 1099\u2003mL (range, 390\u20102450\u2003mL). Initial and residual mean RBCs volumes were 309.9 and 4.0\u2003mL corresponding to a depletion of 98.6\u2003\u00b1\u20030.78\u2003percent. Before processing, the mean numbers of nucleated cells, granulocytes, CD3+ cells, CD34+ cells, and CFU\u2010GM were 20.28\u2003\u00d7\u200310<jats:sup>9<\/jats:sup>, 12.79\u2003\u00d7\u200310<jats:sup>9<\/jats:sup>, 1.96\u2003\u00d7\u200310<jats:sup>9<\/jats:sup>, 356.7\u2003\u00d7\u200310<jats:sup>6<\/jats:sup>, and 195.6\u2003\u00d7\u200310<jats:sup>5<\/jats:sup>, respectively. The mean corresponding recoveries after processing were 33.66, 48.98, 82.02, 82.2, and 93.9\u2003percent. Limited side effects were observed in 14 patients without correlation with residual RBCs volume. All but two patients engrafted.<\/jats:p><jats:p><jats:bold>CONCLUSION:\u2002<\/jats:bold> BM processing with the Cobe Spectra cell separator provides high rates of RBC depletion without significant side effects after BMT.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2006.00735.x","type":"journal-article","created":{"date-parts":[[2006,1,23]],"date-time":"2006-01-23T12:41:39Z","timestamp":1138020099000},"page":"398-402","source":"Crossref","is-referenced-by-count":31,"title":["ABO\u2010mismatched marrow processing for transplantation: results of 114 procedures and analysis of immediate adverse events and hematopoietic recovery"],"prefix":"10.1111","volume":"46","author":[{"given":"Jerome","family":"Larghero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Rea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helene","family":"Esperou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Biscay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Noelle","family":"Maurer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Noelle","family":"Lacassagne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Ternaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Traineau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karima","family":"Yakouben","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Dosquet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Soci\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliane","family":"Gluckman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Benbunan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Pierre","family":"Marolleau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,1,20]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.8.2726"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-01-0339"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1989.29189101156.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1989.29589284141.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1999.39111212.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1089\/152581600319658"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1526-0968.2001.005001046.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1702852"},{"key":"e_1_2_6_10_2","first-page":"4609","article-title":"Immunohematologic 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