{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T09:10:06Z","timestamp":1790068206800,"version":"4.0.1"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:p><jats:bold>BACKGROUND:\u2002<\/jats:bold> Universal leukodepletion (LD) has been implemented in the United Kingdom to reduce the risk of transfusion\u2010transmitted variant Creutzfeldt\u2010Jakob disease. If LD causes microvesiculation of blood cells, however, potentially infectious membrane\u2010associated prion could reach the final products.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:\u2002<\/jats:bold> We have measured microvesicles (MV) derived from red cells (RBC\u2010MV), platelets (PLT\u2010MV), and white blood cells (WBC\u2010MV) and cellular prion protein (PrP<jats:sup>c<\/jats:sup>) in blood components produced by four whole\u2010blood, five RBC, three PLT, and two plasma LD filters and three plateletpheresis techniques.<\/jats:p><jats:p><jats:bold>RESULTS:\u2002<\/jats:bold> RBC\u2010MV and PLT\u2010MV were either unaltered or reduced by all processes, with PLT\u2010MV reduced 10\u2010fold by RBC LD and greater than 300\u2010fold by plasma LD. WBC\u2010MV were reduced or unchanged by RBC and PLT LD and reduced by plasma LD. Whole\u2010blood filtration appeared to increase MVs derived from granulocytes, but the load in the final components was comparable to that in processed RBCs in additive solution. PrP<jats:sup>c<\/jats:sup> was reduced by whole\u2010blood, RBC, and plasma LD and unchanged by PLT techniques. There were differences between various filters and techniques, which were generally minor compared to the overall effects.<\/jats:p><jats:p><jats:bold>CONCLUSION:\u2002<\/jats:bold> These findings suggest no detrimental effects of LD processes in terms of generation of MVs or PrP<jats:sup>c<\/jats:sup> release.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2006.00737.x","type":"journal-article","created":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T11:59:16Z","timestamp":1141214356000},"page":"407-417","source":"Crossref","is-referenced-by-count":31,"title":["The effects of leukodepletion on the generation and removal of microvesicles and prion protein in blood 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