{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:32:01Z","timestamp":1789205521549,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,2,13]],"date-time":"2006-02-13T00:00:00Z","timestamp":1139788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:p><jats:bold>BACKGROUND:\u2002<\/jats:bold> Concern about the safety of blood, blood components, and plasma\u2010derived products with respect to prions has increased since the report of two blood\u2010related infections of variant Creutzfeldt\u2010Jakob disease in the United Kingdom. Efforts were directed toward the development of procedures able to remove or inactivate prions from blood components or plasma\u2010derived products with brain fractions of transmissible spongiform encephalopathy (TSE)\u2010infected rodents as spiking materials. These spiking materials, however, are loaded with pathological prion protein (PrP<jats:sup>TSE<\/jats:sup>) aggregates that are likely not associated to blood infectivity. The presence of these aggregates may invalidate these studies.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:\u2002<\/jats:bold> Brains from 263K scrapie\u2013infected hamsters were suspended in 10\u2003percent phosphate\u2010buffered saline. After low\u2010speed centrifugation, the supernatant was collected and ultracentrifuged at 220,000\u2003\u00d7\u2003<jats:italic>g<\/jats:italic> at 25\u00b0C for 30\u2003minutes. The high\u2010speed supernatants (S<jats:sup>HS<\/jats:sup>) and pellets were collected; the proteinase\u2010resistant PrP<jats:sup>TSE<\/jats:sup> was measured by Western blot and infectivity by intracerebral inoculation into weanling hamsters.<\/jats:p><jats:p><jats:bold>RESULTS:\u2002<\/jats:bold> A substantial amount of prion infectivity (more than 10<jats:sup>5<\/jats:sup> LD<jats:sub>50<\/jats:sub> per mL of a 10% suspension of brain tissues) is present in the S<jats:sup>HS<\/jats:sup> fraction of 263K scrapie\u2013infected hamster brains. Concomitantly, this fraction contains none or only traces of PrP<jats:sup>TSE<\/jats:sup> in its aggregate form.<\/jats:p><jats:p><jats:bold>CONCLUSION:\u2002<\/jats:bold> This study describes a simple and fast protocol to prepare infectious material from 263K scrapie\u2013infected brains that is not contaminated with PrP<jats:sup>TSE<\/jats:sup> aggregates. This S<jats:sup>HS<\/jats:sup> fraction is likely to be the most relevant material for endogenous spiking of human blood in validation experiments aimed at demonstrating procedures to remove or inactivate TSE infectious agents.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2006.00763.x","type":"journal-article","created":{"date-parts":[[2006,2,13]],"date-time":"2006-02-13T09:22:10Z","timestamp":1139822530000},"page":"652-658","source":"Crossref","is-referenced-by-count":34,"title":["Preparation of soluble infectious samples from scrapie\u2010infected brain: a new tool to study the clearance of transmissible spongiform encephalopathy agents during plasma fractionation"],"prefix":"10.1111","volume":"46","author":[{"given":"Vito Angelo","family":"Berardi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco","family":"Cardone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelina","family":"Valanzano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Pocchiari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,2,13]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15486-X"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16811-6"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2004.048660"},{"key":"e_1_2_7_5_2","volume-title":"The National Creutzfeldt\u2010Jakob Disease Surveillance Unit [homepage on the Internet]."},{"key":"e_1_2_7_6_2","volume-title":"The European and Allied Countries Collaborative Study Group of CJD (EUROCJD) [homepage on the Internet]."},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11085-3"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dyh374"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2004.036137"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1580"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.moh.0000143393.49202.f0"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1741-6892.2004.00443.x"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1741-6892.2004.00444.x"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0041-1132.2003.00586.x"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01313752"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(99)00135-4"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.61.12.3688-3693.1987"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)05403-4"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-68-1-219"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/160.5.795"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1998.38998408999.x"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-83-11-2897"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16812-8"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1006\/biol.2002.0327"},{"key":"e_1_2_7_25_2","doi-asserted-by":"crossref","unstructured":"LeeDC StenlandCJ MillerJLC et al.A direct relationship between the partitioning of the pathogenic prion protein and transmissible spongiform encephalopathy infectivity during the purification of plasma proteins [published erratum appears in Transfusion 2001;41:1079]. 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