{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T10:36:03Z","timestamp":1722854163117},"reference-count":8,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,9,4]],"date-time":"2006-09-04T00:00:00Z","timestamp":1157328000000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p><jats:bold>BACKGROUND:\u2002<\/jats:bold> Mass smallpox vaccination with live vaccinia virus has been considered as a preventive measure to counter bioterrorism involving smallpox. This has raised concerns about the possibility of vaccinia virus being transmitted from vaccinated blood donors to recipients. The results of this study could be used to define an appropriate deferral period for blood donors (vaccinated against smallpox) to ensure safety of the blood supply.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:\u2002<\/jats:bold> A procedure was developed to culture vaccinia virus from plasma and peripheral blood mononuclear cells (PBMNCs) of vaccinees enrolled in three smallpox vaccine clinical trials. A total of 665 plasma and PBMNC samples were obtained from 95 vaccinated subjects.<\/jats:p><jats:p><jats:bold>RESULTS:\u2002<\/jats:bold> Vaccinia viremia was not detected by virus culture from plasma and PBMNC samples of healthy vaccinees 3 to 56\u2003days after vaccination under our assay conditions. Plasma viremia assay had a sensitivity of approximately 66 plaque\u2010forming units per mL with a Vero cell culture assay.<\/jats:p><jats:p><jats:bold>CONCLUSION:\u2002<\/jats:bold> The results of this study present evidence that in the case of mass vaccination, the risk of transmission of vaccinia virus by blood transfusion would likely be low.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2006.00936.x","type":"journal-article","created":{"date-parts":[[2006,9,4]],"date-time":"2006-09-04T14:58:40Z","timestamp":1157381920000},"page":"1589-1592","source":"Crossref","is-referenced-by-count":5,"title":["Absence of detectable viremia in plasma and peripheral blood mononuclear cells from smallpox vaccinees: implications for blood safety"],"prefix":"10.1111","volume":"46","author":[{"given":"Kumar","family":"Srinivasan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pradip\u2003N.","family":"Akolkar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf\u2003E.","family":"Taffs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Indira\u2003K.","family":"Hewlett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,9,4]]},"reference":[{"key":"e_1_2_7_2_2","volume-title":"Guidance for industry: recommendations for deferral of donors and quarantine and retrieval of blood and blood products in recent recipients of smallpox vaccine (vaccinia virus) and certain contacts of smallpox vaccine recipients [monograph on the Internet].","year":"2002"},{"key":"e_1_2_7_3_2","first-page":"6.16.1","volume-title":"Current protocols in molecular biology.","author":"Earl PL","year":"1998"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.16.10397-10407.2005"},{"key":"e_1_2_7_5_2","first-page":"271","article-title":"[Studies on postvaccinal encephalitis: behaviour of the vaccinia virus in humans in normal and disturbed vaccination course]","volume":"115","author":"Herzberg\u2010Kremmer H","year":"1930","journal-title":"Zentralbl Bakteriol"},{"key":"e_1_2_7_6_2","first-page":"271","article-title":"[Studies on postvaccinal encephalitis. behaviour of the vaccinia virus in humans in normal and disturbed vaccination course]","volume":"115","author":"Herzberg\u2010Kremmer H","year":"1930","journal-title":"Zentralbl Bakteriol"},{"key":"e_1_2_7_7_2","first-page":"271","article-title":"[Studies on postvaccinal encephalitis: behavior of the vaccinia virus in humans in normal and disturbed vaccination course]","volume":"115","author":"Herzberg\u2010Kremmer H","year":"1930","journal-title":"Zentralbl Bakteriol"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/381101"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.026781"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1537-2995.2006.00936.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1537-2995.2006.00936.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T16:37:26Z","timestamp":1697301446000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1537-2995.2006.00936.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9]]},"references-count":8,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2006,9]]}},"alternative-id":["10.1111\/j.1537-2995.2006.00936.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1537-2995.2006.00936.x","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9]]}}}