{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T07:49:43Z","timestamp":1782460183367,"version":"3.54.5"},"reference-count":27,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2006,11,29]],"date-time":"2006-11-29T00:00:00Z","timestamp":1164758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold>The deletion of three adjacent nucleotides in an exon may cause the lack of a single amino acid, while the protein sequence remains otherwise unchanged. Only one such in\u2010frame deletion is known in the two<jats:italic>RH<\/jats:italic>genes, represented by the<jats:italic>RHCE<\/jats:italic>allele<jats:italic>ceBP<\/jats:italic>expressing a \u201cvery weak e antigen.\u201d<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:<\/jats:bold>Blood donor samples were recognized because of discrepant results of D phenotyping. Six samples came from Switzerland and one from Northern Germany. The molecular structures were determined by genomic DNA nucleotide sequencing of<jats:italic>RHD<\/jats:italic>.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold>Two different variant D antigens were explained by<jats:italic>RHD<\/jats:italic>alleles harboring one in\u2010frame triplet deletion each. Both single\u2010amino\u2010acid deletions led to partial D phenotypes with weak D antigen expression. Because of their D category V\u2010like phenotypes, the<jats:italic>RHD<\/jats:italic>(Arg229del) allele was dubbed<jats:italic>DVL\u20101<\/jats:italic>and the<jats:italic>RHD<\/jats:italic>(Lys235del) allele<jats:italic>DVL\u20102<\/jats:italic>. These in\u2010frame triplet deletions are located in GAGAA or GAAGA repeats of the<jats:italic>RHD<\/jats:italic>exon\u20035.<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold>Partial D may be caused by a single\u2010amino\u2010acid deletion in RhD. The altered RhD protein segments in DVL types are adjacent to the extracellular loop 4, which constitutes one of the most immunogenic parts of the D antigen. These RhD protein segments are also altered in all DV, which may explain the similarity in phenotype. At the nucleotide level, the triplet deletions may have resulted from replication slippage. A total of nine amino acid positions in an Rhesus protein may be affected by this mechanism.<\/jats:p>","DOI":"10.1111\/j.1537-2995.2006.01046.x","type":"journal-article","created":{"date-parts":[[2006,11,29]],"date-time":"2006-11-29T19:49:38Z","timestamp":1164829778000},"page":"2156-2161","source":"Crossref","is-referenced-by-count":21,"title":["In\u2010frame triplet deletions in<i>RHD<\/i>alter the D antigen 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